Differential modulation of dendritic cell (DC) maturation and associated signal transduction as the pivotal events for leprosy spectral pathology

Differential modulation of dendritic cell (DC) maturation and associated signal transduction as the pivotal events for leprosy spectral pathology - Dendritic cells associated with the leprosy spectrum

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON32238

Enrollment

Registered

2008-11-12

Start date

2008-03-01

Completion date

Unknown

Last updated

2024-05-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

immune response leprosy

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: i) Leprosy patients not on leprosy multi-drug treatment ii) healthy contacts of leprosy patients

Exclusion criteria

Exclusion criteria: i) HIV, ii) Other concomitant infections, iii) Multi-drug treatment (MDT) for leprosy or treatment for leprosy reactions, iv) Anti-inflammatory treatment for any other medical condition.

Design outcomes

Primary

Measure	Time frame
The following endpoints will be assayed: i) For maturation of DCs: Increase/decrease in percentage of CD83+ cells in patients as compared to healthy controls after exposure to M.leprae and its antigenic fractions as measured by flow cytometry. ii) For functional activity of DCs: Increased/decreased proliferation of allogeneic T-cells on co-culture with M.leprae antigen-pulsed DCs in a mixed * lymphocyte reaction (MLR) using 3H thymidine incorporation as a readout. iii) For T-helper (Th) polarizing capacity of DCs: Measurement of characteristic Th1 (IFN-g), Th2 (IL-4, IL-5) and Treg (IL-10, TGF-b) in supernatants of M.leprae matured DC primed autologous naïve T cells by ELISA/RT-PCR.	—

Measure

Time frame

The following endpoints will be assayed: i) For maturation of DCs: Increase/decrease in percentage of CD83+ cells in patients as compared to healthy controls after exposure to M.leprae and its antigenic fractions as measured by flow cytometry. ii) For functional activity of DCs: Increased/decreased proliferation of allogeneic T-cells on co-culture with M.leprae antigen-pulsed DCs in a mixed * lymphocyte reaction (MLR) using 3H thymidine incorporation as a readout. iii) For T-helper (Th) polarizing capacity of DCs: Measurement of characteristic Th1 (IFN-g), Th2 (IL-4, IL-5) and Treg (IL-10, TGF-b) in supernatants of M.leprae matured DC primed autologous naïve T cells by ELISA/RT-PCR.

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Secondary

Measure	Time frame
i) For DC receptor identification: Blocking receptors like TLR2, DC-SIGN with specific antibodies and looking at the effect on maturation of DCs as indicated in 7.1.1	—

Countries

Netherlands

Outcome results