chronic inflammation chronic obstructive pulmonary disease (COPD)
Conditions
Interventions
According to the cross-over design, the study comprises two intervention
periods of one week which are separated by a wash-out period of three weeks.
Subjects will participate in a one-week run-in p
Sponsors
Technologiestichting STW (NWO)
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Male subjects in an age of 40-70 years BMI > 20 kg/m2 and 3mg/l No acute and/or chronic inflammatory condition such as arthritis, arthrosis, chronic colitis, etc. during three months before entry of the study No respiratory tract infection or exacerbation of COPD for at least 8 weeks prior to the start of the study No change in treatment regime of the COPD subjects for at least 8 weeks prior to the start of the study Normal constant dietary eating habits and a usual coffee consumption of at least 3 cups per day Non-smokers and stopped smoking, respectively
Exclusion criteria
Exclusion criteria: Women age 70 years old BMI = 30 kg/m2 Diastolic blood pressure (DBP) 90 mmHg, systolic blood pressure (SBP) 150 mmHg Acute and/or chronic inflammatory condition such as arthritis, arthrosis, chronic colitis, etc. during three months before entry of the study Respiratory tract infection or exacerbation of COPD during the last 8 weeks prior to the start of the study Change in treatment regime of the COPD subjects during the last 8 weeks prior to the start of the study Irregular eating habits, usual coffee consumption of less than 3 cups per day and current smoker
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary objective of the present study is to investigate the effects of a one-week daily supplementation of COPD patients with 2 x 250mg caffeine on biomarkers of systemic inflammation in blood such as C-reactive protein (CRP) and the cytokines TNF-a, IL-6, IL-8 and IL-10. | — |
Secondary
| Measure | Time frame |
|---|---|
| The secondary objectives of the study are 1) to investigate the effects of the one week caffeine supplementation on activation of poly-(ADP-ribose) polymerase I (PARP-I) and DNA-repair in peripheral blood lymphocytes by means of immune histochemical methods and the COMET assay. The COMET assay determines the level of DNA strand breaks, and is indicative for the amount of oxidative DNA lesions. 2) to investigate the effects of the one week caffeine supplementation on oxidative stress markers in urine such as the ratio of uric acid/allantoin (the oxidative metabolite of uric acid) and the creatinine levels 3) to determine the levels of caffeine and its metabolites in plasma and urine 4) to investigate the effects of the caffeine supplementation on organic volatiles (e.g. ethane, pentane and other (unknown) compounds that might be discriminatory for COPD patients) in exhaled air 5) to investigate the effects of the caffeine supplementation on the cytokine levels (TNF-a, IL-6, IL-8 and IL-10) in whole blood stimulated ex vivo with LPS. 6) to investigate the effects of the caffeine supplementation on the gene expression levels of cytokines, redox enzymes and other proteins involved in the inflammatory and oxidative stress response | — |
Countries
Netherlands
Outcome results
None listed