De uitkomsten van deze studie gaan gebruikt worden voor een geplande vervolgstudie naar het effect van cacaopolyfenolen op de bloeddruk. Deze biobeschikbaarheidsstudie heeft zelf niet op een aandoening betrekking. high blood pressure Hypertension
Conditions
Interventions
On 5 days in the 3 weeks trial period the volunteers will randomly consume one
of five soy or yoghurt based test drinks with or without cocoa extract. At the
end of the study the volunteers will hav
Sponsors
Academisch Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Male Age 18-70 years Body Mass Index (BMI) 19-30 kg/m2 Reported alcohol consumption
Exclusion criteria
Exclusion criteria: Females Being an employee of the AMC A recorded history or current metabolic diseases, chronic gastrointestinal disorders, cardiovascular or renal disease Currently on a medically prescribed diet, or slimming diet Reported intense sporting activities > 10 hours/week Subjects who receive medical treatment Any surgery in the past 6 months Use of systemic antibiotics in the past 3 months Recent blood donation i.e. 1 month prior to the study and no planned blood donation during the study period Known intolerance or allergy to cocoa, soy or lactose Reported weight change ± 10% the past 6 months Participation in another biomedical study 3 months before the start or during the study The habit of smoking during the past year Consuming meat and/or fish less than twice a week Participation in night shift work
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Two types of body fluids will be collected, i.e. urine and blood. We will focus in plasma on the kinetic profiles of catechins by using HPLC and LC-MS techniques. The 24-hour urine samples contain accumulated metabolites in relatively high concentrations. We will measure several phenolic metabolites by using GC-MS. | — |
Secondary
| Measure | Time frame |
|---|---|
| The effects of cocoa polyphenols on the plasma lipid profile and glucose will be evaluated. Total cholesterol, LDL-C, HDL-C, TG and glucose will be measured by quantitive clinical chemical analysis using the Hitachi 912 analyzer. | — |
Countries
Netherlands
Outcome results
None listed