Co-culturing of primary chondrocytes and mesenchymal stem cells to optimize cartilage formation

Co-culturing of primary chondrocytes and mesenchymal stem cells to optimize cartilage formation - INSTRUCT Technology

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON31506

Enrollment

Registered

2008-04-01

Start date

2009-02-09

Completion date

Unknown

Last updated

2024-05-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Cartilage damage cartilage degeneration

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: -Male and female patients over 18 years of age -Patients with a ruptured ACL admitted to ACL reconstructive surgery

Exclusion criteria

Exclusion criteria: -Patients over 40 years of age -Patients with generalized cartilage degeneration

Design outcomes

Primary

Measure	Time frame
Primary endpoint of this study will be after 6 weeks of in vitro culture or in vivo survival of the co-cultured chondrocytes and mesenchymal stem cells. At this time point we will analyze the ideal ratio of these cells for cartilage formation based on alcian blue staining, collagen propeptide I and II determination, safranin O staining and aggrecan ELISA.	—

Secondary

Measure	Time frame
-	—

Countries

Netherlands

Outcome results