anti-oxidative HDL function
Conditions
Interventions
None listed
Sponsors
Universitair Medisch Centrum Utrecht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Cases: PCOS+, BMI >=27, HOMA >=1.5, 25-40 years of age Controls: PCOS-, BMI
Exclusion criteria
Exclusion criteria: Diabetes mellitus Oral contraceptives Lipid lowering medication Pregnancy Familial hypercholesterolemia Hypertriglyceridemia Clinically manifest cardiovascular disease
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Difference in anti-oxidative capacity of HDL between obese PCOS women with reduced insulin sensitivity and lean controls with normal insulin sensitivity. During oxidation of LDL conjugated dienes are released. Addition of HDL can inhibit the rate of oxidation of LDL; as a consequence less conjugated diens are being released. In the current study, the difference in conjugated dienes between PCOS and controls will be compared. NB the anti-oxidative capacity of HDL is measured and expressed independently of the total amount of HDL | — |
Secondary
| Measure | Time frame |
|---|---|
| Difference in composition of HDL between obese PCOS women with reduced insulin sensitivity and lean controls with normal insulin sensitivity. The composition of HDL particles. Using ultracentrifugation subfractions of HDL will be isolated: HDL2a,2b,3a,3b,3c. In these fractions cholesterol, triglycerides, phospholipids and apolipoproteins will be determined. The composition of these subfractions will be compared between PCOS and controls. As an exploratory parameter the number of endothelial progenitor cells will be determined in PCOS and in controls. | — |
Countries
Netherlands
Outcome results
None listed