The application of buffy coats for histamine release assays.

The application of buffy coats for histamine release assays. - Buffy coats application

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON30126

Enrollment

Registered

2007-01-23

Start date

2006-06-01

Completion date

Unknown

Last updated

2024-06-18

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Allergy IgE mediated Type 1 hypersensitivity

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Healthy male buffy coat donors without IgE sensitization. Hb levels >8.5 mmol/l are required.

Exclusion criteria

Exclusion criteria: Buffy coat donors whose basophils show 1) a high spontaneous histamin release or 2) no histamin release after application of stimuli (non-responders).

Design outcomes

Primary

Measure	Time frame
Primary study parameters: Titers specific IgE (as can be determined in RAST and CAP) do/do not correlate with histamin release when homologous allergens are applied. The biological activity of a purified natural allergen does/does not correlate with a modified/recombinant allergen.	—

Countries

Netherlands

Outcome results