Immunological determinants of human embryo implantation: an ex vivo model

Immunological determinants of human embryo implantation: an ex vivo model - Immunological factors of implantation

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON29908

Enrollment

280

Registered

2006-10-30

Start date

2007-04-01

Completion date

Unknown

Last updated

2024-06-18

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

implantation infertility

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: IVF or ICSI treatment

Exclusion criteria

Exclusion criteria: None

Design outcomes

Primary

Measure	Time frame
The surplus embryos will be cultured on a layer of endometrial stromal cells. The process of adhesion and invasion will be closely followed by measurements of cytokine and trophic factors in culture media, which will provide information on stromal invasion. Main study parameters will be the extent of trophoblast invasion and the cytokine and trophic factor profile of daily produced affluent. Study endpoint will be day 10 of embryo development.	—

Measure

Time frame

The surplus embryos will be cultured on a layer of endometrial stromal cells. The process of adhesion and invasion will be closely followed by measurements of cytokine and trophic factors in culture media, which will provide information on stromal invasion. Main study parameters will be the extent of trophoblast invasion and the cytokine and trophic factor profile of daily produced affluent. Study endpoint will be day 10 of embryo development.

—

Secondary

Measure	Time frame
- The correlation of cytokine/trophic factor patterns expressed in (autologous co-) culture media from normal and abnormal stromal invading embryos. - To investigate whether the chromosomal constitution of the embryo is related to a specific pattern of cytokine expression and implantation.	—

Countries

Netherlands

Outcome results