excessive scarring hypertrophic scarring
Conditions
Interventions
None listed
Sponsors
Universitair Medisch Centrum Groningen
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: In this study 35 healthy women will be included who will be operated for breast-reduction surgery. The technique used must result in an infra-mammary scar. The patients will be older than 18 years of age.
Exclusion criteria
Exclusion criteria: Not healthy enough to undergo the operation. Pregnant women.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| At two days and six weeks the scars, lateral, medial, left and right, will be investigated for complications like hematoma, infection or dehiscence. At three months and a year clinically the height of the scars will be scored as normal, hypertrophic (raised above skin level, but within the confines of the original lesion) or keloid (raised above skin level, but beyond the confines of the orignal lesion). The width of the scar will be measured with a ruler and width and height will be studied intradermally with ultrasound (10 MHz). Standardized pictures will be made at 85 cm of the scar. The investigations will be done on a standard measuring point at three centimer of the outer border of every scar. | — |
Secondary
| Measure | Time frame |
|---|---|
| At two days, three months and a year scar biopsies will be taken form the lateral scars. They will be snap frozen and stored in - 80C for further usage. The biopsies will be sectioned and stained with antibodies. Epidermis: MIB-1 against Ki-67 to score keratinocyte proliferation, Ks8.12 against cytokeratin 16 to score keratinocyte differentiation and CD-1A against Langerhans cells. Epidermis and dermis: antilibodies against TGF-β1,2,3, PDGF, IL-1a, IL-4 en IGF-1,2. These growth factors are associated with excessive scar formation. If the clinical results are hopefull extra research will be done on messenger RNA expression from the different growth factors. | — |
Countries
Netherlands
Outcome results
None listed