Healthy volunteers.
Conditions
Interventions
Helium application before forearm ischemia.
Sponsors
dr. B. Preckel, MD, MA, DEAA, Department of Anesthesiology, Academic Medical Center
University of Amsterdam, Meibergdreef 9
Postbus 22660 H1Z-120, 1100 DD Amsterdam
The Netherlands
Phone: +31 20 5662162, +31 20 5662533 (Secr.)
Fax:+31 20 6979441, e-mail : b.preckel@amc.uva.nl
Eligibility
Inclusion criteria
Inclusion criteria: Healthy volunteers aged 18-65 years, informed consent.
Exclusion criteria
Exclusion criteria: 1. Alcohol consumption; 2. untreated arterial hypertension; 3. known renal impairment; 4. liver disease; 5. cardiovascular disease (arterial hypertension, angina, previous myocardial infarction, history of stroke); 6. history of diabetes mellitus; 7. coagulatopathy (PTT > 1.5 times control); 8. anti coagulation drugs; 9. lipid-lowering drugs; 10. antihypertensive drugs; 11. antioxidant vitamins intake and known hypersensitivity; 12. contra indication or intolerance to any used substance.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The present application of helium seeks to investigate the possibility to precondition human endothelium with helium. This will be done by measurement of forearm blood flow using the venous occlusion plethysmography model. Primary end-point is blood flow and reactive hyperemia before and after ischemia reperfusion after stimulation with either serotonin as an endothelium-dependent vasodilator or sodium nitroprusside as an endothelium-independent vasodilator. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary parameters will be measurement as endothelial adhesion molecules on leukocytes. | — |
Contacts
Public ContactB. Preckel
Department of Anesthesiology, Academic Medical Center University of Amsterdam, Meibergdreef 9 Postbus 22660 H1Z-120
Outcome results
None listed