obesity
Conditions
Interventions
In this study a group of 12 obese individuals and a group of 12 lean individuals (control group) will be exposed to 60 hours of starvation. Before and after this intervention we will obtain muscle bio
Sponsors
Leiden University Medical Center
Eligibility
Inclusion criteria
Inclusion criteria: 1. Healthy males and females; 2. Age 19-60 yrs; 3. Obese subjects: BMI >30 kg/m2; 4. Lean controls: BMI 19-25 kg/m2; 5. Stable weight for the last 3 months; 6. Caucasian; 7. FPG 7.5 mmol/l; 11. Negative family history (first degree) of DM2.
Exclusion criteria
Exclusion criteria: 1. Use of medication known to affect glucose metabolism (for example prednisone) or lipid metabolism; 2. History of genetic or psychiatric disease (e.g. fragile X syndrome, major depression) that affects the brain; 3. Significant chronic disease; 4. Renal or hepatic disease; 5. Pregnancy; 6. Smoking (current); 7. Alcohol consumption of more than 14 units per week at present or in the past; 8. Difficult accessible veins for insertion of an intravenous catheter; 9. Recent blood donation (within the last 3 months); 10. Recent participation in other research projects (within the last 3 months), participation in 2 or more projects in one year; 11. Contra-indication to MRI scanning: A. Claustrophobia; B. Pacemakers and defibrillators; C. Nerve stimulators; D. Intracranial clips; E. Intraorbital or intraocular metallic fragments; F. Cochlear implants; G. Ferromagnetic implants.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary outcome are biochemical parameters (eg AMPK, Sirtuins) measured in muscle biopsies. These will be obtained before and after 60 hours of starvation and for the obese group after the weight loss intervention as well. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary outcomes are: Antropometrics, fMRI, heart rate variability, biometric impedance analysis, blood pressure and pulse, indirect calorimetry and blood samples. All will be obtained before and after 60 hours of starvation and for the obese group after the weight loss intervention as well. | — |
Contacts
Public ContactM.A. Wijngaarden
Outcome results
None listed