Detection of infection with SELDI-tof in case of endophthalmitis.

Determination of protein profiles in vitreous of eyes with endophthalmitis with Surface Enhanced Laser desorption/ ionization time-of-flight (SELDI-tof) technology.

Status

Recruiting

Phases

Unknown

Study type

Interventional

Source

NL-OMON

Registry ID

NL-OMON28646

Enrollment

110

Registered

2009-04-19

Start date

2009-05-01

Completion date

Unknown

Last updated

2024-02-28

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

SELDI-tof,endophthalmitis, vitreous, macular pucker, macular hole.

Interventions

All patients (treatment and control) will undergo vitreous biopsy for this study to collect vitreous for analyses with SELDI-tof. 0.1 ml vitreous will be kept and frozen at -80 degrees Celsius. Additi

Eligibility

Inclusion criteria

Inclusion criteria: Patients with endophthalmitis: 1. Able to give informed consent; 2. Age > 18 years; 3. Initiation of endophthalmitis within 6 weeks after cataract surgery. Controll group: 1. Able to give informed consent; 2. Age > 18 years; 3. Patient that undergo a pars plana vitrectomy because of a macular hole or macular pucker.

Exclusion criteria

Exclusion criteria: Endophthalmitis group: 1. Patients unable to give informed consent; 2. Previous uveitis in the affected eye; 3. Previous pars plana vitrectomy in the affected eye. Controll group: 1. Macylar hole/ macular pucker; 2. Patients unable to give informed consent; 3. Retinopathy because of Diabetes Mellitus or previous uveitis in the affected eye; 4. Previous pars plana vitrectomy in the affected eye.

Design outcomes

Primary

Measure	Time frame
Three analyses will be performed with SELDI-tof in comparison to regular culture methods: 1. Comparison of protein composition of vitreous in comparison to serum obtained bij surgery for maucular hole or macular pucker: controll population; 2. Comparison of protein composition of vitreous in comparison to serum of patients with endophthalmtis; 3. Comparison of protein composition of vitreous in comparison to serum of patients with endophthalmitis versus the controll population.	—

Measure

Time frame

Three analyses will be performed with SELDI-tof in comparison to regular culture methods: 1. Comparison of protein composition of vitreous in comparison to serum obtained bij surgery for maucular hole or macular pucker: controll population; 2. Comparison of protein composition of vitreous in comparison to serum of patients with endophthalmtis; 3. Comparison of protein composition of vitreous in comparison to serum of patients with endophthalmitis versus the controll population.

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Secondary

Measure	Time frame
1. Evaluation of the correlation between specific bacterial causes of endophthalmitis indentified by culture and specific protein pattern obtained via SELDI-tof analyses; 2. Evaluation of the correlation of vitreous of endophthalmitis with a negative result with culture and the protein pattern obtained by SELDI-tof analyses. We will compare the protein patterns of culture negative results with peak pattern of bacteriae identified by culture.	—

Measure

Time frame

1. Evaluation of the correlation between specific bacterial causes of endophthalmitis indentified by culture and specific protein pattern obtained via SELDI-tof analyses; 2. Evaluation of the correlation of vitreous of endophthalmitis with a negative result with culture and the protein pattern obtained by SELDI-tof analyses. We will compare the protein patterns of culture negative results with peak pattern of bacteriae identified by culture.

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Contacts

Public ContactC. Bennebroek

Postbus 85500

C.Bennebroek@umcutrecht.nl+31 (0)88 7551683

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)