gallstones, NAFLD, Liver disease
Conditions
Interventions
None listed
Sponsors
AMC
Eligibility
Inclusion criteria
Inclusion criteria: - 18-65 years of age - BMI < 30 kg/m2 - Individual should be able to give informed consent
Exclusion criteria
Exclusion criteria: - Type 2 diabetes mellitus - Prior bariatric surgery - Inflammatory bowel disease - Primary lipid disorder - Known genetic basis for insulin resistance or glucose intolerance - Ethanol intake > 2 U/week - Pregnancy, females who are breastfeeding - Hepatitis B and/or C - Liver cirrhosis - Auto-immune hepatitis - Wilson disease3/ alpha 1-antitripsine deficiency - Hemochromatosis
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1.Presence of NAFLD/NASH parameters in liver biopsy using histology (NASH-CRN / Steatosis Activity and Fibrosis Score Steatosis) and liver RNA sequencing. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Presence of bacterial DNA, histology and inflammatory genes (RNA seq) liver and abdominal adipose tissue depots as well as metabolites in plasma, feces and urine 2. Fecal and oral microbiota composition 3. Dietary, psychological and satiety lists and excreted metabolites 4. Clinical data (body weight, body composition), waist circumference and blood pressure 5. Genomic DNA (buffy coat) | — |
Contacts
Public ContactStijn Meijnikman
Amsterdam UMC locatie AMC
Outcome results
None listed