Transfusion related acute lung injury Healthy Volunteers Storage Transfusion
Conditions
Interventions
All volunteers will receive i.v. injection of lipopolysaccharide (LPS) 2 ng/kg and 1 unit (300ml) of fresh red blood cells or 1 unit (300 ml of stored red blood cells or 300ml of saline.
Sponsors
Academic Medical Center
Eligibility
Inclusion criteria
Inclusion criteria: 1. Healthy male volunteer 2. Age ≥ 18 years <35 years
Exclusion criteria
Exclusion criteria: 1. No informed consent 2. Any abnormal test result during the screening prior to inclusion of the study (medical history, physical examination, ECG, blood and urine examination, spirometry, chest x-ray). 3. History of drugs abuse 4. Any present medication use on prescription 5. Smoking < 6 months 6. History of blood donation < 3 months 7. Previously transfused 8. Participation in any other medical study < 3 months 9. Participated in previous volunteer studies using LPS
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Onset of lung injury measured by laboratory measurements, functional assays, radiological imaging, pulmonary function tests. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Markers of inflammation and coagulation 2. Identification of potential bio-markers for diagnosing TRALI | — |
Contacts
Public ContactA.L. Peters
Department of Intensive Care Medicine and LEICA Meibergdreef 9
Outcome results
None listed