COVID-19
Conditions
Interventions
Blood taking procedure (total amount of 22.1 mL blood)
Sponsors
ZonMw
Eligibility
Inclusion criteria
Inclusion criteria: - Age of 18 years or older - A suspected SARS-CoV-2 virus infection - Need for oxygen supplementation - CRP level > 50 mg/L - D-dimer level > 0.5 mg/L - Ability to provide written informed consent
Exclusion criteria
Exclusion criteria: - History of venous thromboembolism - Use of anticoagulant therapy at inclusion - Known hereditary or acquired thrombophilia
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Percentage of endothelial surface in the blood-vessel-on-chip model that is covered by platelets and fibirn ("clotting") upon treatment with COVID-19 patient plasma. Coverage in the blood-vessel-on-chip will be measured by fluorescence microscopy. | — |
Secondary
| Measure | Time frame |
|---|---|
| Several measurements in the patient plasma: - d-dimer level - thrombin-antithrombin complexes (TAT) - cytokine levels ((IL-1ß, IL-6, and TNF-a) - Markers of neutrophil extracellular traps (NETs) using ELISA: a) Myeloperoxidase (MPO)/DNA b) Citrullinated histone H3 - Tissue factor (TF) expressing extracellular vesicles as measured by a TF-dependent factor Xa activity assay. | — |
Contacts
Public ContactThijs van Haaps
Amsterdam UMC loc. AMC
Outcome results
None listed