The effects of single leg immobilization on muscle protein synthesis, protein breakdown, and glucose uptake in healthy young men

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON26917

Enrollment

Registered

2016-09-30

Start date

2016-11-01

Completion date

Unknown

Last updated

2024-02-28

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Immobilization, muscle wasting

Interventions

Day 0 Upon arrival to the laboratory in the fasted state, a saliva and blood sample will be taken. Participants will then ingest heavy water that allows for measuring integrated muscle protein synthe

Eligibility

Inclusion criteria

Inclusion criteria: 1) Healthy males, between the ages of 18 and 30 y 2) BMI between 18.5 and 30.0 kg/m2 3) Able and willing to provide informed consent

Exclusion criteria

Exclusion criteria: 1) The use of anti-inflammatory and/or analgesic medication, whether it is prescription or not 2) A history of neuromuscular disorders or muscle/bone wasting diseases 3) Any acute or chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, insulin-dependent or insulin-independent diabetes, or the presence of any other metabolic disease – all of which will be determined via a medical history screening questionnaire 4) The use of any medications known to affect protein metabolism (glucocorticoids, non-steroidal anti-inflammatory medication, or prescription strength acne medication, etc.) 5) A (family) history of thrombosis 6) The use of anticoagulant medications 7) Consumption of tobacco-containing products 8) Excessive alcohol consumption (>21 units/wk) 9) History of bleeding diathesis, platelet or coagulation disorders, or antiplatelet/anticoagulation therapy

Design outcomes

Primary

Measure	Time frame
1)The effect of single leg immobilization on basal and postprandial skeletal muscle protein synthesis 2)The effect of single leg immobilization on basal and postprandial skeletal muscle protein breakdown 3)The effect of single leg immobilization on rates of skeletal muscle glucose uptake	—

Secondary

Measure	Time frame
1) The effect of single leg immobilization on muscle mass, muscle strength, and body composition 2) The effect of single leg immobilization on integrated (chronic) rates of muscle protein synthesis	—

Contacts

Public ContactStefan Gorissen

Department of Kinesiology - McMaster University

E-mail: gorisses@mcmaster.ca+1 905 730 5894

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)