Assisted reproductive technologies help overcoming many causes of infertility, and are widely used all over the world. Unfortunately efficiencies of the current technologies remain relatively low with pregnancy rates of only 20-30% per embryo transfer. This may be due to the format of the culture (static drops covered with oil), culture parameters (e.g. medium composition), extensive manipulation of the embryos and the inability to identify the most viable embryo. Therefore new approaches are ne
Conditions
Interventions
Frozen-thawed human embryos are either cultured in standard culture dishes or in microfluidic systems which enable the collection of essential information on the development of the embryos.
Sponsors
VUmc (VU University Medical Center)
Eligibility
Inclusion criteria
Inclusion criteria: Frozen-thawed donated human embryos of sufficient morphological quality will be included in the study. The following criteria have to be met after thawing: 1. Minimum number of blastomeres: 8 cells; 2. Maximum degree of fragmentation: 20%; 3. Maximum degree of atresia: 25%.
Exclusion criteria
Exclusion criteria: Embryos with insufficient morphological quality after thawing will be excluded from the study according to the criteria described above.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The main study endpoint is the blastocyst formation rate on day 5, 28 hours after thawing, in both, the control and experimental group. | — |
Secondary
| Measure | Time frame |
|---|---|
| The secondary study parameter is the morphology of each embryo at different time points. The percentage of embryos that reach each developmental stage will be recorded and analysed as secondary study endpoints. Furthermore we will assess how many embryos are suitable for embryo transfer one day after thawing according to standard laboratory criteria. | — |
Contacts
Public ContactD.C. Kieslinger
Amstelveenseweg 601
Outcome results
None listed