Composition of IVF culture media affects birth weight by differential epigenetic effects in placental tissue.

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON26382

Enrollment

128

Registered

2011-01-17

Start date

2011-02-01

Completion date

Unknown

Last updated

2024-02-28

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

subfertility, epigenetics

Interventions

Two commercially available culture media are used for the embryo culture in IVF treatment.

Eligibility

Inclusion criteria

Inclusion criteria: 1. Patients pregnant after IVF or IVF with ICSI where one of the two tested culture media systems is used (i.e. participants in trial NTR1979); 2. Only pregnancies following fresh transfer; 3. Only singleton pregnancies.

Exclusion criteria

Exclusion criteria: Usage of donor gametes.

Design outcomes

Primary

Measure	Time frame
Rate of methylation and expression of imprinted genes. Gene expression will be analysed using a quantitative real-time PCR method. For the methylation analysis, the DNA will be treated with bisulphite and subsequently pyrosequencing will be used to quantitatively analyse the methylation level of the regions of interest.	—

Secondary

Measure	Time frame
Correlation between methylation and expression of imprinted genes and birth weight.	—

Contacts

Public ContactA. Montfoort, van

Maastricht University Medical Center Dept. of Obstetrics and Gynaecology Postbus 5800

aafke.van.montfoort@mumc.nl+ 31 43 3872012

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)