None
Conditions
Interventions
Sponsors
None
Eligibility
Inclusion criteria
Inclusion criteria: - Male - Age 18-35
Exclusion criteria
Exclusion criteria: - Participation in a scientific intervention study in the last 3 months - No informed consent - History of coagulation disorders - Active use of prescription medication - Use of anticoagulant medication, including aspirin - History of liver disease - History of chronic transmittable disease - History of alcohol, smoking or drug abuse
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| ROTEM lysis, EXTEM/FIBTEM/ABTEM (i.e. LT, LOT, Li (t), ML, CLR), measured on the day of the blood draw | — |
Secondary
| Measure | Time frame |
|---|---|
| ADAMTS13 plasma determinations (antigen, activity, conformation, degradation) Plasma turbidity (time to onset, slope, degree) Whole blood clot lysis, using HALO assay (time to onset, slope, degree), Platelet activation (CD62P, PAC1), using flow cytometry Platelet adhesion and aggregation, using flow model (Time to platelet adhesion, aggregation and breakdown of clots, measured using fluorescent live-cell imaging). *There will be only one blood draw (and thus one timepoint) for the volunteer. All outcomes except the ADAMTS plasma determinations. will be measured on the day of thid blood draw. The ADAMTS13 plasma determinations will be performed on a later moment on frozen plasma obtained from the inital blood draw. | — |
Contacts
Public ContactPieter Sloos
Amsterdam UMC, location AMC
Outcome results
None listed