Sleep
Conditions
Interventions
One night of short sleep (4h) will be compared to one night of normal sleep (8h).
Sponsors
Leiden University Medical Center
Eligibility
Inclusion criteria
Inclusion criteria: - Informed consent - Male Caucasian - Age between 18 and 55 years - BMI between 20 and 35 kg/m2
Exclusion criteria
Exclusion criteria: - Active endocrine disease (e.g. diabetes mellitus type 1 and type 2, thyroid disease, any signs of Cushing’s syndrome, adrenal disease and lipid-associated disorders such as FH) - Fasting glucose >7.0 mmol/L - Severe chronic disease (e.g. chronic liver or kidney disease) - Severe insomnia, sleep disorders or exceptional habitual sleep duration (10 h). - Medication use including the following: lipid lowering drugs, glucocorticoids, sleep medication, hormone replacement, glucose lowering drugs, insulin therapy, antidepressants and psychotropic drugs (last 6 weeks)), anti-coagulants - Recent time zone travel (last 6 weeks) - Shift work (last 6 weeks) - Severe alcohol use (>21 units/week) - Psychiatric disease - Drug abuse - Recent participation to another nutritional or biomedical trial (last 6 weeks) - Medication use which may interfere with study measurements, as judged by the responsible physician - Reported weight loss or weight gain (10%) in the last six months prior to the pre-study screening - Clinically relevant abnormalities in clinical chemistry at screening (to be judged by the study physician) - Reported use of any nicotine containing products in the six months preceding the study and during the study itself; - Extreme strenuous exercise during last 3 months, as judged by responsible physician - Excessive sunbathing during last 3 months, as judged by responsible physician.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| - Metabolic flexibility; measured as change in RQ and lipid levels after a standard meal - Stress responsiveness; measured as change in stress hormones, heart rate and blood pressure after a mild physical stressor | — |
Secondary
| Measure | Time frame |
|---|---|
| - Tissue ageing; measured in skin - Intracellular metabolic pathways; measured in subcutaneous white fat and skeletal muscle tissue - Circadian rhythm in hormone and skin temperature rhythms | — |
Contacts
Public ContactRosa van den Berg
Albinusdreef 2 / 2333 ZA Leiden
Outcome results
None listed