healthy, young subjects
Conditions
Interventions
Subjects will receive a nutritional supplement during immobilisation and recovery. This will be either a protein hydrolysate or a placebo
Sponsors
Nuritas
Eligibility
Inclusion criteria
Inclusion criteria: 1) Male 2) Aged 18-35 y 3) BMI 18.5-30.0 kg/m2
Exclusion criteria
Exclusion criteria: 1) (Family) history of thrombosis 2) (Family) history of Factor V Leiden, or other known thrombophilia (such as; protein C, protein S, antithrombin deficiency) 3) Lower limb, back or shoulder injuries (which may interfere with the use of crutches) 4) Allergies to milk protein 5) Lactose intolerance 6) Participation in structured resistance exercise program 7) All co-morbidities interacting with mobility and muscle metabolism of the lower limbs (e.g., arthritis, spasticity/rigidity, all neurological disorders and paralysis) 8) Any medications known to (or may) affect protein metabolism (i.e., corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications) 9) Diagnosed diabetes 10) Diagnosed metabolic, cardiovascular or intestinal disorders 11) A history of neuromuscular problems 12) Use of anti-coagulants 13) Use of protein and/or fish-oil supplements 14) Participation in a 2H2O study in the previous 6 months. 15) Smoking 16) Any recent hospital admission/ major surgery
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| skeletal muscle mass (quadriceps muscle cross sectional area (CSA)) | — |
Secondary
| Measure | Time frame |
|---|---|
| maximal leg muscle strength (1RM), whole-leg muscle CSA, type I and II muscle fibre size, muscle protein synthesis rates, and muscle signalling and gene transcription responses | — |
Contacts
Public ContactMichelle Weijzen
Maastricht University
Outcome results
None listed