Metabolism, vitamin B2 status
Conditions
Interventions
None listed
Sponsors
Wageningen University & Research
Eligibility
Inclusion criteria
Inclusion criteria: - 18-28 year old female - BMI 18.5-25 kg/m2 - VO2max = 37 mL/kg/min or VO2max = 47 mL/kg/min; - Performed a valid VO2max test In order for the test to be considered valid two out of three of the following conditions should be met: 1) The maximal heart rate is within 10 beats of the predicted maximum (220 – age) 2) A plateau in in VO2 was reached; VO2 fails to increase with 150 mL/min, despite an increase in work load 3) Respiratory exchange ratio (RER) = 1.00 has been achieved
Exclusion criteria
Exclusion criteria: • Health concerns regarding respiratory and pulmonary diseases, for example COPD or (exercise induced) asthma • (Known symptoms of) chronic diseases, for example cardiovascular disease and cancer; • (Known symptoms of) metabolic diseases, for example type I or II diabetes or hyperthyroidism; • (Known symptoms of) inborn metabolic diseases, for example glucose-6-phosphate dehydrogenase (G6PD) deficiency; • (Known symptoms of) hematological disorders, for example anemia or disturbed red blood cell formation; • Haemoglobin concentrations below 7.5 mmol/L; • Regular smoker (defined as smoking >5 cigarettes per week); • Lactating or pregnant; • Following a veganistic lifestyle, i.e. excluding the consumption of animal or animal-derived food products. Vegetarians are allowed to participate in the study. • Usage of hormonal contraceptive medications other than the birth control pill Microgynon 20/30 or a generic variant containing 0.02/0.03 mg ethinylestradiol and 0.10/0.15 mg levonorgestel. Examples include IUD contraceptives or contraceptive rings. The use of condoms or pessaria is allowed; • Recent use (within four months) of supplements with suggestive training effects, for example creatine phosphate, EPO or anabolic steroid; • Recent daily usage (within four weeks) of supplements containing vitamin B2 (including multivitamin supplements) and usage of vitamin B2 supplements during the study; • Usage of recreational drugs, for example marihuana, amphetamines and cocaine during the study (starting after first screening day); • Suffers from (sport) injury that hampers maximal exercise performance; • Blood donation during the previous 2 months or during the course of study; • Current participation in other clinical trials; • Not subscribed to a general practitioner (GP) practice; • Employed or undertaking a thesis or internship at the department of Human and Animal Physiology.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary outcome is to study mitochondrial function in PBMCs from endurance-trained and untrained young females and link it to vitamin B2 status. | — |
Secondary
| Measure | Time frame |
|---|---|
| • To compare basal and exercise-stimulated mitochondrial function in PBMCs to analyse how exercise impacts mitochondrial function and whether this differs between endurance-trained and untrained young females; • To analyse vitamin B2 status in cells, 24-hour urine and plasma in basal and exercise-stimulated endurance-trained and untrained young females to understand how exercise impacts vitamin B2 status and whether this differs between endurance-trained and untrained females; • To characterize muscle mitochondrial capacity in endurance-trained and untrained young females using NIRS measurements to study the association between vitamin B2 status and muscle mitochondrial function; • To investigate the mechanistic link between mitochondrial function in PBMCs and vitamin B2 status by analysing markers of mitochondrial and metabolic health in plasma, cells and 24-hour urine. | — |
Contacts
Public ContactJoëlle Janssen
Wageningen University & Research
Outcome results
None listed