Sepsis, ICU-acquired weakness, muscle wasting,
Conditions
Interventions
A 5 day enteral deuterated water dosing protocol will be conducted during ICU admission to measure diurnal rates of muscle protein synthesis. Patients will receive 400ml of 70 mol% enriched deuterated
Sponsors
Maastricht UMC+ (azM)
Eligibility
Inclusion criteria
Inclusion criteria: 1) Age >18 5 days
Exclusion criteria
Exclusion criteria: 1) Coagulation disturbances (not including use of anti-coagulants in prophylactic dosages) 2) Contraindication to enteral infusion (e.g. due to GI-tract perforation) 3) Any trauma resulting in severe injury or fracture of any extremity. 4) Rhabdomyolysis 5) Proven (pre-existing) skeletal muscle weakness (e.g. due to neuromuscular disorders or immobility) 6) Renal dysfunction defined as a serum creatinine >171 mol/L or a urine output of less than 500 ml/last 24 hours 7) Patients requiring chronic veno-venous hemofiltration 8) Patients on any form of extracorporeal life support (ECMO/ELS) 9) Weight less than 50 kg or greater than 100 kg 10) Pregnant patients or lactating with the intent to breastfeed 11) Previous enrollment in this study 12) Previous participation in a 2H amino acid tracer study within the last year
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Fractional synthesis rates (FSR) of muscle protein using muscle protein-bound and plasma 2H-alanine enrichments | — |
Secondary
| Measure | Time frame |
|---|---|
| - changes in leg skeletal muscle mass (ultrasound of rectus femoris CSA) - urinary 3-methylhistidine concentrations - transcriptional changes of genes involved in muscle protein synthesis and breakdown | — |
Contacts
Public ContactRob van Gassel
P.O. Box 616,
Outcome results
None listed