The effects of protein dose response on myofibrillar and mitochondral protein synthesis after endurance exercise in young men

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

NL-OMON

Registry ID

NL-OMON24647

Enrollment

Registered

2015-03-23

Start date

2015-04-01

Completion date

Unknown

Last updated

2024-06-11

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Young males, Muscle protein synthesis

Interventions

exercise bout and protein drink with different doses

Eligibility

Inclusion criteria

Inclusion criteria: - Males - Aged between 18-35 years - Bodyweight between 65-95 kg inclusive - Healthy, moderately trained - BMI < 30 kg/m2 - Having given informed consent

Exclusion criteria

Exclusion criteria: - Having any identified metabolic or intestinal disorders - Use of tobacco products - Non-steroidal anti-inflammatory drugs (NSAID) in the 4 days prior to the experimental trial - Allergies to milk proteins (whey or casein) - Arthritic conditions - A history of neuromuscular problems - Previous participation in amino acid tracer studies - Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications). - Diabetes - Training more than 4 days per week - VO2max 70 ml/kg/min - Phenylketonuria - lactose intolerance - blood donation within two months of study initiation

Design outcomes

Primary

Measure	Time frame
Muscle protein synthesis rate (myofibrillar and mitochondrial)	—

Secondary

Measure	Time frame
• The fractional synthetic rate (FSR) of muscle protein synthesis (myofibrillar, and mitochondrial proteins) from 0-3 and 3-6 hours in the post-prandial period. • Plasma free L-[ring-2H5]-phenylalanine enrichment (expressed as MPE) • Plasma free L-[ring-2H4]-tyrosine enrichment (expressed as MPE) • Plasma free L-[3,5-2H2]-tyrosine enrichment (expressed as MPE) • Plasma free L-[ring-2H5]-phenylalanine enrichment • Plasma free L-[1-13C]-leucine enrichment • Plasma free L-[1-13C]-KIC enrichment • Plasma total phenylalanine (expressed as ìmol/L) • Plasma total tyrosine (expressed as ìmol/L) • Total plasma amino acids (AAmax [ìmol/L]) • Plasma glucose (glucosemax [mmol/L]) • Plasma insulin (insulinmax [mU/L])	—

Measure

Time frame

• The fractional synthetic rate (FSR) of muscle protein synthesis (myofibrillar, and mitochondrial proteins) from 0-3 and 3-6 hours in the post-prandial period. • Plasma free L-[ring-2H5]-phenylalanine enrichment (expressed as MPE) • Plasma free L-[ring-2H4]-tyrosine enrichment (expressed as MPE) • Plasma free L-[3,5-2H2]-tyrosine enrichment (expressed as MPE) • Plasma free L-[ring-2H5]-phenylalanine enrichment • Plasma free L-[1-13C]-leucine enrichment • Plasma free L-[1-13C]-KIC enrichment • Plasma total phenylalanine (expressed as ìmol/L) • Plasma total tyrosine (expressed as ìmol/L) • Total plasma amino acids (AAmax [ìmol/L]) • Plasma glucose (glucosemax [mmol/L]) • Plasma insulin (insulinmax [mU/L])

—

Contacts

Public ContactT.A. Churchward-Venne

Department of Human Movement Sciences - Maastricht University Medical Centre+

t.churchward-venne@maastrichtuniversity.nl+31 43 388 2154

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)