atherosclerosis innate immune memory high-fat challenge slagaderverkalking geheugen van het aangeboren afweersysteem vetrijke maaltijd
Conditions
Interventions
A single high-fat challenge (milkshake containing 95g of fat) and ‘control’ shake (comparable to an average breakfast).
Sponsors
Radboud University Medical Center
Eligibility
Inclusion criteria
Inclusion criteria: - Age between 18 and 40 years - LDL cholesterol < 3.5 mmol/l, fasting triglycerides < 2 mmol/l - No previous cardiovascular events
Exclusion criteria
Exclusion criteria: - Smoking within the year before study entry - Diagnosed with any long-term medical condition that can interfere with the study (i.e. renal failure, cardiovascular disease, diabetes, rheumatoid arthritis etc.) - Medication (with the exception of oral contraceptives) or supplement use (i.e. omega3) - BMI 27 kg/m2 - Previous vaccination within 3 months prior to study entry - Current infection or clinically significant infections within 1 month before study entry (defined as fever > 38.5°C) - Allergic to cow milk/dairy products - Pregnancy/lactation
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary endpoint is the TNFa production upon ex vivo stimulation with LPS of monocytes isolated 72h after the consumption of high-fat shake. | — |
Secondary
| Measure | Time frame |
|---|---|
| Additional secondary endpoints are the production of other cytokines and chemokines upon ex vivo stimulation, the monocytes’ inflammatory phenotype as assessed by flowcytometry analysis , epigenetic and metabolic reprogramming and serum induced inflammation. | — |
Contacts
Public ContactJulia van Tuijl
Radboudumc
Outcome results
None listed