Healthy population Food allergies (Voedselallergie) Intestinal permeability (Darmdoorlaatbaarheid) Detection method development for dietary protein in blood (Ontwikkeling detectivemethode voor voedingseiwit in bloed)
Conditions
Interventions
Intake of 100grams of roasted peanuts.
Blood sampling via venflon cannula at baseline and 30, 60, 120, 240, and 360 min after peanut intake.
Sponsors
Wageningen University
Eligibility
Inclusion criteria
Inclusion criteria: - 20-35 year old males and females - Body mass index (BMI) 18.5-25 k- Suitable veins for blood sampling
Exclusion criteria
Exclusion criteria: - History of peanut allergy - Known symptoms of immune disease, such as diabetes, gastritis, and coeliac disease. - Known symptoms of intestinal disease, such as Crohn’s Disease, ulcerative colitis, and irritable bowel syndrome. - Smoking - Use of hard drugs - Use of specific medicines: - Chronic use of NSAIDs: aspirins, ibuprofen, etc. - Drugs having an effect on gastric and/or intestinal function and motility, including antidepressants. - Participation in other scientific studies - Blood donation during the last six weeks before the start of the study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The main study outcome is the number of subjects in which Ara h6 can be detected in serum over time following peanut consumption with a sandwich ELISA. | — |
Secondary
| Measure | Time frame |
|---|---|
| The secondary outcome is sensitivity of the sandwich ELISA after further optimization | — |
Contacts
Public ContactLonneke Janssen Duijghuijsen
Wageningen UR Food and Biobased Research
Outcome results
None listed