Rabies
Conditions
Interventions
None
Sponsors
LUMC
Eligibility
Inclusion criteria
Inclusion criteria: In order to be eligible to participate in this study, a hyperimmunized subject must meet all of the following criteria: - 18 years old or older. - Received at least 3 rabies vaccinations. - Able to provide informed consent. A control subject must meet the following criteria: - 18 years old or older. - Never received rabies vaccinations. - Able to provide informed consent.
Exclusion criteria
Exclusion criteria: A potential subject who meets any of the following criteria will be excluded from participation in this study: - History of (pre)syncope associated with medical procedures involving needles. - Received any vaccination other than rabies three months prior to inclusion. - Administration of plasma or blood products three months prior to inclusion. - Bleeding disorders or use of anticoagulants. - Any current infectious disease other than seasonal cold. - Immunocompromised (due to medication, medical condition, or other).
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| An optimal combination of concentrations of rabies virus and rabies-specific antibodies will be used to detect and quantify the population of rabies-specific B-cells using flow cytometry. We expect to see no rabies-positive B-cell events in unvaccinated individuals. If we see a rabies-positive B-cell population in hyperimmunized individuals, we can conclude that our assay is able to detect rabies-specific B-cells, which is our primary endpoint. This outcome will be measured in blood drawn from the participants via venipuncture at one single timepoint. This timepoint is independent of the most recent rabies vaccination. | — |
Secondary
| Measure | Time frame |
|---|---|
| In an explorative setting, we want to see if we can quantify the population of rabies-specific B-cells in different donors, using flow cytometry. Furthermore, we would like to explore if we can further classify the rabies-specific population into different B-cell subsets.This outcome will be measured in blood drawn from the participants via venipuncture at one single timepoint. This timepoint is independent of the most recent rabies vaccination. | — |
Contacts
Public ContactLisanne Overduin
LUMC
Outcome results
None listed