hypoxia altitude thrombosis thrombin generation
Conditions
Interventions
Induction of hypoxia by either going on altitude or by a stay in a low oxygen chamber.
Sponsors
Maastricht University
Eligibility
Inclusion criteria
Inclusion criteria: • Healthy subjects (not having any morbidity and without obvious sign of illness and not taking any medication interfering with coagulation) willing to participate to both parts of our study. • All healthy volunteers will undergo a physical check-up by an authorized medical doctor. During this physical check-up the doctor will look at the ECG, the blood pressure, the heart rate and blood oxygen level. • Between 18 and 50 years of age. We take 50 years as a maximal age to prevent any co-morbidity that could have an influence on coagulation (like diabetes, atherosclerosis, peripheral arterial disease, …).
Exclusion criteria
Exclusion criteria: • Subjects taking any medication interfering with coagulation. • Subjects having a cardiovascular disease or any other serious medical problem. • Subjects below 18 or above 50 years of age.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Thrombin generation will be done in whole blood and plasma with and without the addition of exogenous thrombomodulin. The parameters we will study at are: the peak height, ETP, lagtime, time-to-peak and velocity index. Samples after TG will be fixated for scanning electron microscopy (SEM) for cell morphology. Facs experiments will be performed for PS-exposure and platelet reactivity. Flow experiments for thrombus formation analysis with microfluidic chips. | — |
Secondary
| Measure | Time frame |
|---|---|
| • Routine coagulation tests (APTT, PT, INR, platelet count, haematocrit) • Measurements of coagulations factor levels (II, V, VII, VIII, IX, X, protein S and C, hemoglobin) • Blood pressure, heart frequency, blood oxygen level. | — |
Contacts
Public ContactM. Ninivaggi
Outcome results
None listed