Brown adipose tissue, Cold acclimation, Skeletal muscle, Non-shivering, Thermogenesis, Diabetes, Obesity
Conditions
Sponsors
Maastricht University Medical Center + (MUMC+)
Eligibility
Inclusion criteria
Inclusion criteria: General 1. Male 2. BMI 28-35 3. Age 18-35 for obese and 18-65 for diabetics 4. Caucasians Obese: 5. Fasting glucose < 6.1 mmol/l 6. No sings of active cardiovascular disease, uncontrolled hypertension, liver or kidney malfunction Type 2 diabetics: 1. Diagnosed with type 2 DM at least 1.5 years before start of the study 2. Well-controlled T2DM (HbA1C < 8.5%) 3. No sings of active diabetes-related co-morbidities like cardiovascular disease, diabetic foot, polyneuropathy, retinopathy 4. No signs of active uncontrolled hypertension, liver or kidney malfunction
Exclusion criteria
Exclusion criteria: 1. Participate in physical activity more than 2x/week 2. Participation in earlier research that included a PET/CT-scan 3. Radiation therapy due to medical treatment 4. Unstable body weight 5. Participation in another biomedical study within 1 month before the first screening visit 4. Use of Beta-blockers; Type 2 diabetics: 1. Insulin dependent diabetic subjects
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Standard uptake values (SUVs) of FDG of active brown adipose tissue with the PET/CT-scans, one time prior and one time post the cold acclimation period (2x 2hour); 2. Energy expenditure with indirect calorimetry, one time prior and one time post the cold acclimation period (3x 3hour); 3. Skeletal muscle mitochondrial uncoupling with the muscle biopsy, one time before and one time post the cold acclimation period (2x 30minutes). BRITE cell recruitment with the fat biopsy, one time prior and one time post the cold acclimation period (2x 30minutes). 4. Glucose infusion rate (ml/h) by hyperinsulinemic euglycemic clamp, one time prior and one time post the cold acclimation period (2x 5hour) | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Body temperatures (core and skin temperatures) with iButtons and a telemetric pill, one time prior and one time post the cold acclimation period; 2. Skin perfusion with laser Doppler flowmetry, one time prior and one time post the cold acclimation period; 3. Blood parameters, among others insulin, glucose, free fatty acids and thyroid hormones, one time prior and post the cold acclimation period; 4. Body composition with a DXA-scan, one time prior and post the cold acclimation period. | — |
Contacts
Public ContactAnouk Lans, van der
Universiteitssingel 50
Outcome results
None listed