Diabetes, Insulin resitance and mitochondrial dysfunction
Conditions
Interventions
Infusion with with to different lipid emulsion respectivily Intralipid® and Clinoleic®, versus Saline. Each patient is his own control.
Sponsors
Department of Endocrinology and Metabolism, Academic Medical Center Amsterdam, the Netherlands.
Eligibility
Inclusion criteria
Inclusion criteria: 1. Healthy male volunteer 2. Caucasian 3. Age between 18-35 years 4. Normal BMI ( 20-24.9 kg/m2) 5. VO2 max (90-110% predicted value according to Jones formula) 6. Stable weight during 3 months before participation and during participation 7. Normal physical activity with at least 2 times per week sport activities 8. Non-smoking
Exclusion criteria
Exclusion criteria: 1. Fasting blood glucose > 5,6 mmol/L or abnormal OGTT (2-hour value after oral ingestion of 75 g, glucose >7.8 mmol/l) Fasting insulin concentration >50 pmol/l 2. BMI> 25 and BMI< 20 3. DM type II in first degree relatives 4. All chronic diseases 5. Drug use 6. Dyslipidaemia 7. Abnormal liver or renal function
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| - Peripheral glucose uptake (Rd) - Resting, adp-stimulated and uncoupled mitochondrial respiration rates | — |
Secondary
| Measure | Time frame |
|---|---|
| -Skeletal muscle metabolites of plasma FFA, i.e. glycosphingolipids - Plasma concentrations of adiponectin, insulin, cortisol, glucagon, catecholamines | — |
Contacts
Public ContactM. Brands
Academic Medical Center (AMC) F5-162 Endocrinology and Metabolism
Outcome results
None listed