Sepsis Bacteraemia/ bacteremia Molecular diagnosis bacteria in blood
Conditions
Interventions
None listed
Sponsors
VU University Medical Center
Eligibility
Inclusion criteria
Inclusion criteria: - Age >= 18 years ; - Bacteraemia with at least one blood culture with growth of Gram-negative bacteria or Gram-positive cocci in chain observed with microscopy; - Bacteraemia with at least one blood culture with growth of Gram-positive cocci (in clusters) and at least one of the following syndromes suggestive of S. aureus infection: arthritis, wound infection, thrombophlebitis, meningitis, soft tissue infection, vertebral osteomyelitis, splenic abscess or other microbiological evidence for S. aureus infection.
Exclusion criteria
Exclusion criteria: - Blood culture obtained >48 hours; - Intravenous antibiotic therapy >48 hours; - Haematological disorder; - Anaemia (an Hb below 5.5 mmol/L); - Participation in BLENT-study of patients with endocarditis; - Previous participation in this study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Primary objectives: 1.To determine the diagnostic performance of five molecular assays for the detection of bacteraemia in patients. 2.To evaluate the relationship between the bacterial DNA load (BDL), quantitative culture and culture time-to-positivity in patients with bacteraemia. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary objective: 1. To assess the putative applicability of the molecular assays in routine molecular diagnostics with regard to costs, hands on time and difficulty of the protocol in routine use. | — |
Contacts
Public ContactM. Brand, van den
VU University Medical Center Department of Medical Microbiology and Infection Control Van der Boechorststraat 7
Outcome results
None listed