The effect of insulin resistance on brown adipose tissue activity.

The effect of fasting-induced insulin resistance on brown adipose tissue activity, non-shivering thermogenesis and skeletal muscle mitochondrial uncoupling.

Status

Recruiting

Phases

Unknown

Study type

Interventional

Source

NL-OMON

Registry ID

NL-OMON21276

Enrollment

Registered

2012-07-12

Start date

2012-07-01

Completion date

Unknown

Last updated

2024-02-28

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Brown adipose tissue, insulin resistance, non-shivering thermogenesis, skeletal muscle

Interventions

Volunteers will undergo 2 FDG PET/CT scans of the upper body to determine BAT glucose uptake rate during mild cold exposure. One scan will be performed during a normal-fed period (after 6 hours of fas

Eligibility

Inclusion criteria

Inclusion criteria: 1. BMI: 18-25 kg/m2; 2. Age: 18-30 years; 3. For females: specific oral contraceptive; 4. Caucasians; 5. Good general health; 6. Sedentary (<2 times/week or <3 hours/week sports).

Exclusion criteria

Exclusion criteria: 1. Diabetes mellitus; 2. Elevated fasting blood glucose levels (>5.6 mmol/l); 3. Cardiovascular diseases; 4. Use of beta-blockers; 5. Asthma or other obstructive pulmonary diseases; 6. Hyperthyroidism; 7. For females: pregnancy; 8. Participation in earlier research that included a PET/CT scan; 9. Radiation therapy due to medical treatment.

Design outcomes

Primary

Measure	Time frame
1. Standard Uptake Values (SUV) of FDG in active BAT, as measured using FDG PET/CT scans (normal-fed period vs. prolonged fasting period); 2. Energy expenditure, as measured using indirect calorimetry, during thermoneutral and mild cold conditions (normal-fed period vs. prolonged fasting period); 3. Skeletal muscle mitochondrial uncoupling, as measured using high-resolution respirometry, in muscle biopsies (normal-fed period vs. prolonged fasting period); 4. Ex vivo skeletal muscle insulin-stimulated glucose uptake, as a measure of insulin sensitivity (normal-fed period vs. prolonged fasting period).	—

Measure

Time frame

1. Standard Uptake Values (SUV) of FDG in active BAT, as measured using FDG PET/CT scans (normal-fed period vs. prolonged fasting period); 2. Energy expenditure, as measured using indirect calorimetry, during thermoneutral and mild cold conditions (normal-fed period vs. prolonged fasting period); 3. Skeletal muscle mitochondrial uncoupling, as measured using high-resolution respirometry, in muscle biopsies (normal-fed period vs. prolonged fasting period); 4. Ex vivo skeletal muscle insulin-stimulated glucose uptake, as a measure of insulin sensitivity (normal-fed period vs. prolonged fasting period).

—

Secondary

Measure	Time frame
1. Skin temperatures and core temperature during thermoneutral and mild cold conditions (normal-fed period vs. prolonged fasting period); 2. Skin perfusion, using Laser Dopller Flowmetry (normal-fed period vs. prolonged fasting period); 3. Blood parameters, among others insulin, glucose, free fatty acids, catecholamines and thyroid hormones, during thermoneutral and mild cold conditions (normal-fed period vs. prolonged fasting period).	—

Measure

Time frame

1. Skin temperatures and core temperature during thermoneutral and mild cold conditions (normal-fed period vs. prolonged fasting period); 2. Skin perfusion, using Laser Dopller Flowmetry (normal-fed period vs. prolonged fasting period); 3. Blood parameters, among others insulin, glucose, free fatty acids, catecholamines and thyroid hormones, during thermoneutral and mild cold conditions (normal-fed period vs. prolonged fasting period).

—

Contacts

Public ContactM. Hanssen

Universiteitssingel 50

mjw.hanssen@maastrichtuniversity.nl/+31 (0)43 3884259

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)