COPD, Emphysema, mesenchymal stem cells
Conditions
Interventions
1. Central bronchial biopsies of the 1st, 3rd and 5th generation
will be taken from 20 emphysema patients admitted to the
bronchoscopic lung volume reduction program in the UMCG.
2. Lung resection ma
Sponsors
Pathology & Medical Biology and Pulmonology Dept.,
University Medical Center Groningen
Eligibility
Inclusion criteria
Inclusion criteria: Lung resection material will be obtained from COPD patients and nonCOPD controls, who undergo lung transplantation or lobectomy / pneumectomy because of lung cancer. COPD patients will be selected on basis of having smoked more than 20 pack years and having clinical signs of emphysema. Written informed consent will be collected from all patients in order to be eligible for inclusion. The nonCOPD controls will be selected on basis of having smoked less than 1 pack year and having no clinical signs of emphysema. Lung cancer patients who undergo lobectomy / pneumectomy will be selected on basis of the size and location of the tumor, enabling adequate collection of tissue material, without interfering with routine oncopathology procedures.
Exclusion criteria
Exclusion criteria: Patients with alpha1antitrypsin deficiency will be excluded.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Succesfull isolation and culturing of mesenchymal stemcells derived from lung tissue. 2. Succesfull isolation and culture of mesenchymal stemcells derived from adipose tissue. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. Analyses of differential MSC function between COPD and control groups through RTPCR, immunohistochemical stainings and Western Blot. | — |
Contacts
Public ContactN.H.T. Hacken, ten
University Medical Center Groningen (UMCG) Department of Pulmonology
Outcome results
None listed