insulin resistance, inflammation
Conditions
Interventions
Intervention with broccoli seedlings (BroccoCress).
Butter letuce will be used as a control.
Volunteers received 75 gram glucose in 250 mL tap water,
leading to postprandial inflammation as measured
Sponsors
University Medical Center Groningen
Eligibility
Inclusion criteria
Inclusion criteria: 1. Healthy male volunteer aged 18 to 30 and BMI 18-25 kg/m2, extremities included; 2. Not involved in intensive sportive activities more than twice a week (e.g. playing football, tennis, running, race-cycling, swimming); 3. Stable weight and no intention to lose weight until completion of the study; 4. Signed written informed consent form (ICF).
Exclusion criteria
Exclusion criteria: 1. Not being able to fast overnight (10 hours); 2. Vegetarians; 3. Documented Diabetes mellitus or fasting glucose level of >6.1mmol/l at screening; 4. Clinically significant inflammatory disease (possibly interfering with measurement of parameters in this study); 5. Intake of medication (from 2 weeks before screening until the end of the study, except for sporadic use of paracetamol and/or treating an AE); 6. Smoking; 7. Donation of blood within the last 3 months prior to admission to the clinic; 8. Participation to another clinical study within 90 days before enrolment; 9. Positive drug screen or alcohol breath test at D-1; 10. Fear of blood and/or needles; 11. Veins unsuitable for intravenous (i.v.) catheter on either arm (e.g., veins that are difficult to locate, access or puncture, veins with a tendency to rupture during or after puncture); 12. Clinically relevant abnormalities in clinical chemistry, hemoglobin or positive HIV, HbsAg and/or HepC at screening.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The main outcomes of this study are inflammation and insulin sensitivity. Inflammation will be addressed by PBMC activation as measured by nuclear translocation of NF-kappaB, plasma cytokine levels and insulin sensitivity as determined by plasma concentrations of glucose and insulin as well as the uptake of glucose in tissues. | — |
Secondary
| Measure | Time frame |
|---|---|
| Sulforaphane kinetics, measured in plasma and urine. | — |
Contacts
Public ContactM.G. Priebe
University Medical Center Groningen (UMCG) Antonius Deusinglaan 1
Outcome results
None listed