The effect of cold acclimation on brown adipose tissue.

The effect of cold acclimation on human brown adipose tissue and skeletal muscle mitochondrial uncoupling.

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

NL-OMON

Registry ID

NL-OMON20696

Enrollment

Registered

2012-02-28

Start date

2011-12-01

Completion date

Unknown

Last updated

2024-02-28

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Brown adipose tissue Cold acclimation Skeletal muscle Non-shivering thermogenesis

Interventions

The volunteers will undergo two PET/CT-scans, in which cold-induced BAT activity will be measured before and after a cold acclimation period of 10 days. To investigate the role of the skeletal muscle

Eligibility

Inclusion criteria

Inclusion criteria: 1. BMI 18-25; 2. Age 18-30; 3. Females: On oral contraceptive; 4. Caucasians.

Exclusion criteria

Exclusion criteria: 1. Diabetes Mellitus; 2. Females: Pregnancy; 3. Participate in physical activity more than 2x/week; 4. Use of Beta-blockers; 5. Cardiovascular diseases; 6. Asthma or other obstructive pulmonary diseases; 7. Elevated fasting blood glucose level (> 5.6 mmol/L); 8. Participation in earlier research that included a PET/CT-scan; 9. Radiation therapy due to medical treatment.

Design outcomes

Primary

Measure	Time frame
1. Standard uptake values (SUVs) of FDG of active brown adipose tissue with the PET/CT-scans, one time prior and one time post the cold acclimation period (2x 2hour); 2. Energy expenditure with indirect calorimetry, one time prior, half way and one time post the cold acclimation period (3x 3hour); 3. Skeletal muscle mitochondrial uncoupling with the muscle biopsy, one time before and one time post the cold acclimation period (2x 30minutes). BRITE cell recruitment with the fat biopsy, one time prior and one time post the cold acclimation period (2x 30minutes).	—

Measure

Time frame

1. Standard uptake values (SUVs) of FDG of active brown adipose tissue with the PET/CT-scans, one time prior and one time post the cold acclimation period (2x 2hour); 2. Energy expenditure with indirect calorimetry, one time prior, half way and one time post the cold acclimation period (3x 3hour); 3. Skeletal muscle mitochondrial uncoupling with the muscle biopsy, one time before and one time post the cold acclimation period (2x 30minutes). BRITE cell recruitment with the fat biopsy, one time prior and one time post the cold acclimation period (2x 30minutes).

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Secondary

Measure	Time frame
1. Body temperatures (core and skin temperatures) with iButtons and a telemetric pill, one time prior, half way and one time post the cold acclimation period; 2. Skin perfusion with laser Doppler flowmetry, one time prior, half way and one time post the cold acclimation period; 3. Blood parameters, among others insulin, glucose, free fatty acids and thyroid hormones, one time prior, half way and post the cold acclimation period; 4. Body composition with a DXA-scan, one time prior and post the cold acclimation period.	—

Measure

Time frame

1. Body temperatures (core and skin temperatures) with iButtons and a telemetric pill, one time prior, half way and one time post the cold acclimation period; 2. Skin perfusion with laser Doppler flowmetry, one time prior, half way and one time post the cold acclimation period; 3. Blood parameters, among others insulin, glucose, free fatty acids and thyroid hormones, one time prior, half way and post the cold acclimation period; 4. Body composition with a DXA-scan, one time prior and post the cold acclimation period.

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Contacts

Public ContactAnouk Lans, van der

Universiteitssingel 50

a.vanderlans@maastrichtuniversity.nl+31 (0)43 3884259

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)