endometriosis local estrogen metabolism intracrinology
Conditions
Interventions
Blood (10 mL), urine (10 mL) and peritonel fluid sampling (10 mL) prior to standard care (e.g. surgery, medical treatment). Completing a questionnaire about life-style. Obtaining an eutopic endometria
Sponsors
Maastricht University Medical Centre (azM)
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Cases: - Being older than 18 year - Diagnosis of endometriosis (laparoscopically confirmed) - Being scheduled for elective laparoscopic (or laparotomy) surgery for endometriosis - Not taking hormonal medications in the previous three months - Subjects must have signed an approved informed consent Controls: - Being older than 18 year - Being premenopausal - Having no prior diagnosis of endometriosis - Not taking hormonal medications in the previous three months - Being scheduled for any gynaecological surgery like for tubal ligation, for a benign uterine disease, for diagnosis, for laparoscopic hysterectomy and bilateral adnexectomy - Subjects must have signed an approved informed consent
Exclusion criteria
Exclusion criteria: Cases: - Being younger than 18 year - Diagnosis of atypical hyperplasia, other types of cancer - Previous diagnosis of endometrial carcinoma - Being under hormonal medication during the three months preceding the sampling - Pregnancy - Inability to approve the informed consent form Controls: - Being younger than 18 year - Being diagnosed with a benign ovarian diseases - Being diagnosed with any malignancy - Previous diagnosis of endometriosis - Being under hormonal medication during the three months preceding the sampling - Pregnancy - Inability to approve the informed consent form
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Clinical data (via a case report form) and epidemiological, life style and symptoms data (via a questionnaire) will be collected prospectively. Blood samples will be taken before treatment. During surgery endometrial and endometriosis biopsies will be collected. Serum and tissue will be analysed for the steroid profile (LCMS). Biopsies will be assessed for the levels of the enzymes involved in the local estrogen generation. Steroid metabolism data will be correlated with clinical characteristics and symptomatology in each subject. | — |
Secondary
| Measure | Time frame |
|---|---|
| non applicable | — |
Contacts
Public ContactAndrea Romano
Dept. Obstetrics and Gynaecology GROW-School for Oncology and Developmental Biology Maastricht University Medical Centre P. Debyelaan 25
Outcome results
None listed