Oxidative Stress
Conditions
Keywords
Muaythai, Turmeric Extract, Antioxidants, Malondialdehyde, Catalase, Glutathione
Brief summary
This study examined whether taking turmeric extract for 28 days affects oxidative stress and antioxidant defenses in healthy men who practice Muaythai and men who do not exercise regularly. Oxidative stress occurs when reactive molecules exceed the body's protective defenses and can damage cells. The study included 44 healthy men aged 18-25 years in four groups of 11: Muaythai athletes without supplementation, Muaythai athletes taking turmeric extract, men who did not exercise regularly and received no supplementation, and men who did not exercise regularly and took turmeric extract. Participants were first categorized as Muaythai athletes or sedentary individuals according to their physical activity status. Within each category, participants were randomly allocated to the turmeric supplementation group or the control group using a lottery method. The supplementation groups received 400 mg of turmeric extract daily for 28 days. The athlete groups followed a structured Muaythai training program. Blood samples were collected on Day 1 and Day 28 to measure malondialdehyde, a marker of oxidative stress, and catalase and reduced glutathione, which are involved in the body's antioxidant defenses. Changes over time were evaluated within and between groups.
Detailed description
To minimize the acute effects of exercise on oxidative stress and biochemical parameters, all participants underwent a three-day period of complete rest before each blood sampling session. Venous blood samples were collected at baseline (Day 1) and at the end of the intervention (Day 28).
Interventions
DIETARY_SUPPLEMENTTurmeric Extract
Participants in the Muaythai plus turmeric and sedentary plus turmeric groups received one capsule containing 400 mg of turmeric root extract (Solgar), standardized to 93% total curcuminoids (372 mg), once daily after the evening meal for 28 days.
BEHAVIORALStructured Muaythai Training
Participants in both Muaythai athlete groups followed a structured Muaythai training program based on progressive overload for 28 days.
Sponsors
Sinan Ağlar
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Four parallel groups of 11 participants each were studied: Muaythai athletes without supplementation, Muaythai athletes receiving turmeric extract, sedentary men without supplementation, and sedentary men receiving turmeric extract. Supplementation consisted of 400 mg of turmeric extract daily for 28 days. Participants remained in their respective groups throughout the study. Blood samples were collected on Days 1 and 28.
Eligibility
Sex/Gender
MALE
Age
18 Years to 25 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy men aged 18-25 years residing in Van, Türkiye. * Non-smokers and non-drinkers. * Provision of written informed consent. * For the Muaythai groups: regular Muaythai practice for at least eight years, training six days per week, and placement among the top three in a Turkish national championship or selection for the national team. * For the sedentary groups: no sports background and no regular exercise participation.
Exclusion criteria
* Failure to meet the inclusion criteria. * Allergic reactions to turmeric identified during pre-supplementation allergy screening in participants considered for the supplementation groups.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in malondialdehyde (MDA) level | Baseline (Day 1) and Day 28 | Change in blood malondialdehyde (MDA) level from Day 1 to Day 28, measured spectrophotometrically and expressed in µmol/L. |
| Change in catalase (CAT) activity | Baseline (Day 1) and Day 28 | Change in blood catalase (CAT) activity from Day 1 to Day 28, measured spectrophotometrically and expressed in U/mL. |
| Change in reduced glutathione (GSH) level | Baseline (Day 1) and Day 28 | Change in blood reduced glutathione (GSH) level from Day 1 to Day 28, measured spectrophotometrically and expressed in µmol/L. |
Countries
Turkey (Türkiye)
Outcome results
None listed