Colorectal Cancer
Conditions
Keywords
Wilms' tumor 1, Cancer colon, Angiogenesis, Immunohistochemistry
Brief summary
Colorectal carcinoma is a major bublic health problem. It is due to several genetic mutations. Wilms' tumor1 (WT1) gene is a tumor suppressor gene that has been mutated in several human tumors. Detection of WT1 in colorectal carcinoma may provide better future results.
Detailed description
Colorectal adenocarcinoma represents one third of human caners worldwide. It results from cumulative genetic mutations. the incidence of colorectal carcinoma is rising, so, detecting new therapeutic modalities is important. Wilms' Tumor 1 (WT1) gene is a tumor suppressor gene. in certain tumors; WT1 acts as an oncogene, facilitating tumor growth and spread. WT1 is also involved in angiogenesis. we decided to detect expression of WT1 in 60 patients with colorectal adenocarcinoma and detect any association between WT1 expression and certain tumor oarameters as perineural invasion, lymphovascular invasion, lymph node metastasis.
Interventions
BIOLOGICALimmunohistochemical staining with WT1 antibody
4 micrometer thick tissue sections will be stained by anti humal WT1 immunohistochemical antibody.
Sponsors
Sohag University
Study design
Observational model
CASE_ONLY
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* patients with colorectal adenocarcinoma.
Exclusion criteria
* patients who received pre-operative neoadjuvant therapy.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| detection of WT1 in colorectal adenocarcinoma | 6 months | detection of WT1 in colorectal adenocarcinoma and correlate its expression in tumor cells with certain parameters as patients ages, sexes, tumor grade, lymph node metastasis, lymphovascular invasion and perineural invasion. |
Countries
Egypt
Outcome results
None listed