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Effect of Powered Toothbrushing and Dental Flossing Sequence on Dental Plaque Removal

Does the Sequence of Powered Toothbrushing and Dental Flossing Affect Dental Plaque Removal? A Randomized Crossover Clinical Trial

Status
Completed
Phases
Unknown
Study type
Interventional
Source
ClinicalTrials.gov
Registry ID
NCT07737626
Enrollment
46
Registered
2026-07-30
Start date
2026-05-02
Completion date
2026-07-15
Last updated
2026-07-30

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Dental Plaque

Brief summary

This randomized crossover clinical trial evaluated whether the sequence of powered toothbrushing and dental flossing affects dental plaque removal. Forty-six systemically and periodontally healthy participants were randomly assigned to one of two oral hygiene sequences. In Group 1, participants performed powered toothbrushing followed by dental flossing during the first period and dental flossing followed by powered toothbrushing during the second period. Group 2 performed the same procedures in the opposite order. The two intervention periods were separated by a 15-day washout period, with professional prophylaxis performed to standardize oral hygiene conditions. Dental plaque was assessed before and after the oral hygiene procedures using the Plaque Index (PI) and the Turesky modification of the Quigley-Hein Plaque Index (TQHPI). The study aimed to determine whether performing powered toothbrushing before or after dental flossing results in greater plaque removal.

Interventions

BEHAVIORALPowered Toothbrushing

Participants will perform oral hygiene using a powered toothbrush according to the standardized study protocol. The intervention will be applied as part of the assigned brushing-flossing sequence.

BEHAVIORALDental Flossing

Participants will perform interdental cleaning using dental floss according to the standardized study protocol. The intervention will be applied as part of the assigned brushing-flossing sequence.

Sponsors

Lokman Hekim University
Lead SponsorOTHER_GOV

Study design

Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
SINGLE (Outcomes Assessor)

Eligibility

Sex/Gender
ALL
Age
18 Years to 30 Years
Healthy volunteers
Yes

Inclusion criteria

* Age between 18 and 30 years * Systemically healthy * Presence of at least 22 natural teeth * No signs of active periodontal disease * Ability to comply with the study procedures and oral hygiene instructions * Provision of written informed consent

Exclusion criteria

* Antibiotic use within the previous 3 months * Smoking * Presence of systemic conditions known to affect periodontal health, including diabetes mellitus or immune-mediated disorders * Ongoing orthodontic treatment * Presence of fixed prostheses, crowns, or extensive restorations that could interfere with plaque assessment * Professional periodontal therapy within the previous 3 months * Pregnancy or lactation * Severe dental crowding

Design outcomes

Primary

MeasureTime frameDescription
Residual Plaque IndexImmediately after completion of the assigned oral hygiene sequence following 48 hours of plaque accumulation, in each study period.Residual dental plaque was assessed immediately after completion of the assigned oral hygiene sequence using the Plaque Index (PI). Higher scores indicate greater residual plaque accumulation.
Residual Buccal Turesky Modified Quigley-Hein Plaque Index (TQHPI)Immediately after completion of the assigned oral hygiene sequence following 48 hours of plaque accumulation, in each study period.Following plaque disclosure, residual plaque on the buccal tooth surfaces was assessed using the Turesky Modified Quigley-Hein Plaque Index (TQHPI), scored from 0 to 5, with higher scores indicating greater residual plaque accumulation.
Residual Lingual Turesky Modified Quigley-Hein Plaque Index (TQHPI)Immediately after completion of the assigned oral hygiene sequence following 48 hours of plaque accumulation, in each study period.Following plaque disclosure, residual plaque on the lingual tooth surfaces was assessed using the Turesky Modified Quigley-Hein Plaque Index (TQHPI), scored from 0 to 5, with higher scores indicating greater residual plaque accumulation.

Countries

Turkey (Türkiye)

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Jul 31, 2026