Exercise Training, Healthy Adult Participants, Neuromuscular Adaptations, Nutritional Intervention
Conditions
Keywords
exercise, mitochondria, knee extensors, performance
Brief summary
The goal of this study is to investigate the effect of combining olive leaf extract supplementation with endurance training (moderate intensity continuous training or sprint interval training). We hypothesize that OLE supplementation during training, as compared to training alone with placebo, will translate into better performance outcomes. More specifically, we hypothesize that the additional benefits of OLE supplementation on performance will be mediated by improved mitochondrial characteristics in skeletal muscle and increased neural activation.
Detailed description
Maximal exercise capacity (VO2peak) predicts cardiovascular risk and all-cause mortality. Regular moderate to vigorous physical activity, a key determinant of VO2peak, is essential for an optimal health status of the general population. Thus, strategies that accentuate the health benefits of exercise may have considerable therapeutic benefits. Skeletal muscle Ca2+ handling is a primary determinant of exercise capacity. The regulation of skeletal muscle contraction by Ca2+ is coupled to mitochondrial energy production through a mechanism involving transport across the inner mitochondrial membrane by the mitochondrial Ca2+ uniporter (MCU). Ca2+ entry in the mitochondrial matrix controls Ca2+ -sensitive enzymes, including pyruvate dehydrogenase (PDH) activity, a rate-limiting enzyme for glycolysis. Olive leaf extract (OLE) contains \ 40% of the natural polyphenol oleuropein and is a potent activator of MCU in skeletal muscle with anti-inflammatory and antioxidant properties. The present study will be a double-blind, randomized, placebo-controlled, parallel study in healthy young individuals (18-45 years) consisting of a 3-week endurance training (either moderate intensity continuous training or sprint interval training) period combined with OLE or placebo intake. We will examine the impact of OLE supplementation on maximal exercise capacity, knee extensor neuromuscular function, muscular and blood adaptations. The results of this study will elucidate whether OLE supplementation improves fitness parameters and the molecular mechanisms regulating these adaptations.
Interventions
DIETARY_SUPPLEMENTOlive leaf extract
A capsule containing 250 mg of an olive leaf extract preparation will be swallowed with a glass of water daily for 21 days.
DIETARY_SUPPLEMENTPlacebo
A capsule containing 336 mg of cellulose microcrystalline will be swallowed with a glass of water daily for 21 days.
Sponsors
University of Lausanne
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Masking description
The CHUV pharmacy will perform the randomization.
Intervention model description
The design is a double-blind, randomized, placebo-controlled, parallel study.
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
1. Being between 18 and 45 years old 2. Being healthy (assessed through the PAR-Q questionnaire). 3. Being physically active, but not involved in any structured training program 4. Being available for the study period.
Exclusion criteria
1. If a participant answers positively to any YES/NO question in the PAR-Q health questionnaire. For questions requiring a precision, any positive answer will be reviewed by a medical doctor, who will determine the inclusion/exclusion of the participant. 2. Having allergy to xylocaine 3. Being pregnant.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| maximal aerobic power | before and after the three-week training period | maximal power (in watts) measured during a graded exercise to failure |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Maximal oxygen uptake | before and after the three-week training period | maximal oxygen uptake (ml/min/kg) measured during a graded exercise until failure |
| Submaximal heart rate and power | before and after the three-week training period | heart rate and power measured during submaximal cycling exercise |
| Knee extensor neuromuscular function | before and after the three-week training period | maximal voluntary contraction force and maximal voluntary activation level |
Countries
Switzerland
Contacts
CONTACTNicolas Place, PhD
Outcome results
None listed