Effects of Using Miswak in Addition to Oral Hygiene Products in Patients With Gingivitis

Status

Not yet recruiting

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT07689786

Enrollment

Registered

2026-07-08

Start date

2026-07-04

Completion date

2027-07-04

Last updated

2026-07-08

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Gingivitis; Chronic

Keywords

periodontal disease, gingivitis, periodontal inflamation, miswak

Brief summary

This pilot study aims to evaluate the effects of miswak use on the oral biological environment by comparing biochemical parameters obtained from saliva and dental plaque samples collected from individuals who use miswak and controls. A total of 36 healthy volunteers will be enrolled in the study, including 18 miswak+rutine oral hygiene products users and 18 rutine oral hygiene products. Periodontal clinical parameters will be recorded, and saliva and supragingival dental plaque samples will be collected from all participants. The collected samples will be analysed for biomarkers associated with periodontal inflammation and oxidative stress. Interleukin-1 beta (IL-1β), C-reactive protein (CRP), and oxidative stress levels will be measured using biochemical analysis. The findings of this study are expected to provide preliminary evidence regarding the biological effects of miswak on oral health and contribute to the current understanding of its potential role as an adjunctive oral hygiene tool.

Interventions

COMBINATION_PRODUCTMiswak

Participants used miswak (Salvadora persica L.) as an adjunct to toothbrushing, five times per day, for one month before periodontal treatment.

OTHERToothpaste

Participants used a manual toothbrush twice daily for one month before periodontal treatment.

Study design

Observational model

COHORT

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to 60 Years

Healthy volunteers

Yes

Inclusion criteria

* To be in the age range of 18-60 years * To have no systemic disease * To be diagnosed with gingivitis

Exclusion criteria

* Smoking * History of recent periodontal treatment

Design outcomes

Primary

Measure	Time frame	Description
Change in Salivary Interleukin-1 Beta (IL-1β) Levels	Pre-op 4 weeks and after 6 weeks	To evaluate the effect of adjunctive miswak use on salivary interleukin-1 beta (IL-1β) levels in patients with gingivitis. Salivary IL-1β concentrations will be measured using enzyme-linked immunosorbent assay (ELISA).

Secondary

Measure	Time frame	Description
Change in Clinical Periodontal Indices	Pre-op 4 weeks and after 6 weeks	To evaluate changes in clinical periodontal parameters, including Plaque Index (PI), Gingival Index (GI), and Bleeding on Probing (BOP) following adjunctive miswak use.
Change in Salivary C-Reactive Protein (CRP) Levels	Pre-op 4 weeks and after 6 weeks	To evaluate changes in salivary C-reactive protein (CRP) concentrations as a marker of oral inflammatory status following adjunctive miswak use.
Change in Salivary Oxidative Stress Levels	Pre-op 4 weeks and after 6 weeks	To evaluate changes in salivary oxidative stress levels following adjunctive miswak use using appropriate biochemical assays.

Countries

Turkey (Türkiye)

Contacts

CONTACTAysegul Sari, PhD

aysglsr@gmail.com05321732518

CONTACTHasan Burak SEN, res. assist.

buraksenh1999@gmail.comTürkiye

PRINCIPAL_INVESTIGATORHasan Burak Sen, DDs

Hatay Mustafa Kemal Üniversitesi

STUDY_DIRECTORAyşegül Sari, PhD