Cardiorespiratory Fitness, Cortisol, Exercise Physiology, Interleukin-6
Conditions
Keywords
Neuroendocrine, ınflammatory, fitness level, cortisol, exercise
Brief summary
This study aimed to investigate salivary interleukin-6 (IL-6) and cortisol responses following short-term and long-term cycling exercise in team athletes with different cardiorespiratory fitness levels. Participants completed a Wingate anaerobic cycling test and a YMCA submaximal cycling test in a randomized crossover design separated by a seven-day washout period. Saliva samples were collected before exercise, immediately after exercise, and 30 minutes after exercise to determine IL-6 and cortisol concentrations. The study also examined whether cardiorespiratory fitness level influenced the magnitude of these physiological responses.
Detailed description
Exercise-induced physiological stress activates numerous neuroendocrine and inflammatory pathways, including the hypothalamic-pituitary-adrenal axis and cytokine-mediated responses. Cortisol and interleukin-6 (IL-6) are among the most commonly investigated biomarkers of exercise stress and adaptation. However, the influence of cardiorespiratory fitness on acute hormonal and inflammatory responses to exercise remains incompletely understood. This study employed a randomized crossover design involving team athletes aged 18-35 years. Participants completed two cycling exercise protocols: a short-term anaerobic test (Wingate) and a long-term aerobic test (YMCA submaximal cycling test). The two testing sessions were separated by a seven-day washout period and were performed at the same time of day to minimize circadian variation. Saliva samples were collected before exercise, immediately after exercise, and 30 minutes post-exercise. Salivary IL-6 and cortisol concentrations were analyzed to determine acute physiological responses to both exercise modalities. Participants were categorized according to cardiorespiratory fitness level based on estimated VO₂max values, and biomarker responses were compared between fitness groups.
Interventions
BEHAVIORALWingate Anaerobic Cycling Test
Participants performed a 30-second all-out Wingate anaerobic cycling test to evaluate acute physiological responses. Saliva samples were collected before exercise, immediately after exercise, and 30 minutes after exercise for cortisol and interleukin-6 analysis.
BEHAVIORALYMCA Submaximal Cycling Test
Participants performed a YMCA submaximal cycling test to assess cardiorespiratory fitness and exercise-induced physiological responses. Saliva samples were collected before exercise, immediately after exercise, and 30 minutes after exercise for cortisol and interleukin-6 analysis.
Sponsors
Ondokuz Mayıs University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
Team athletes aged 18 to 35 years Participation in organized team sports (e.g., volleyball, basketball, soccer, or handball) Minimum of 3 training sessions per week, with each session lasting at least 1 hour Sports participation history of at least 2 years Apparently healthy and free from known cardiovascular, metabolic, endocrine, or respiratory diseases Willingness to provide written informed consent
Exclusion criteria
Current musculoskeletal injury or illness affecting exercise performance Use of medications known to influence hormonal or inflammatory responses Smoking or use of tobacco products Acute infection within the previous two weeks Failure to complete both exercise testing sessions Failure to comply with pre-test instructions
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Salivary Cortisol Response | Pre-exercise, immediately post-exercise, and 30 minutes post-exercise | Change in salivary cortisol concentrations measured before exercise, immediately after exercise, and 30 minutes after exercise following short-term and long-term cycling tests. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Salivary Interleukin-6 (IL-6) Response | Pre-exercise, immediately post-exercise, and 30 minutes post-exercise | Change in salivary interleukin-6 concentrations measured before exercise, immediately after exercise, and 30 minutes after exercise following short-term and long-term cycling tests. |
Countries
Turkey (Türkiye)
Outcome results
None listed