Professional Caregivers Working With Infants Under Age 6 Months
Conditions
Brief summary
Antimicrobial resistance genes (ARGs) are the genes that allow bacteria to survive antibiotic treatments. These genes can be found in beneficial gut microbes, and occur more frequently in infants than adults. These genes can also transmit between different bacterial species. Exclusively breastfed infants carry fewer ARGs than formula fed infants. We are trying to find out if care-givers working with exclusively breastfed infants have fewer ARGs than those working with formula fed infants.
Interventions
We will collect stool samples and questionnaires on infant caregivers
Sponsors
University of Florida
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
* Employment at a childcare facility or home daycare at time of sampling * Participant works and interacts with infants under the age 6 months old, including responsibility for changing diapers * Participant \>18 years old
Exclusion criteria
* Participant has a history or presence of a medical condition including Ulcerative Colitis, Crohn's Disase, Inflammatory Bowel Disease, colon cancer, Celiac's disease, or other GI disordrs * Current priobitic use or probiotic use within the last 4 weeks * Participant is currently pregnant or breastfeeding * Participant has an infant \<12 months old at home for 3 or more days per week * Enrollment in a clinical trial involving an invstigational drug or dvic * Antibiotic use within the past 3 months * Participant working exclusively with infants \>6 months old at time of stool sample collection
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Count of antimicrobial resistance genes (ARGs) in stool | 2 weeks from enrollment | Count of ARGs in stool sample, and association with infant usual diet |
| Abundance of antimicrobial resistance genes (ARGs) | 2 weeks from enrollment | Abundance of ARGs in stool samples from caregivers in relation to infant usual feeding practices |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Count and diversity of antimicrobial resistance genes (ARGs) and caregiver diet | 2 weeks from enrollment | We will also test for the extent caregiver diet impacts their own carriage of ARGs |
| Diversity and abundance of antimicrobial resistance genes (ARGs) and interactions | 2 weeks | Determine the extent to which caregiver type of interaction (e.g. diaper changes, cuddling infants) impacts caregiver ARGs |
| Caregiver gut microbiome | 2 weeks | Determine the extent to which infant diet, caregiver diet, and infant-caregiver interactions impact the caregiver gut microbiome. |
Contacts
CONTACTDiana H Taft, PhD
CONTACTDylan KP Blaufus, MS
Outcome results
None listed