Immunometabolism, Inflammation, Mitochondrial Function, Bioenergetics
Conditions
Keywords
Nutraceuticals, Exercise, Bioenergetics, Immunometabolism, Inflammation, High intensity interval training
Brief summary
The study consist of 3 interventional periods which will be completed by each consenting participant in a randomized, crossover fashion. The interventional periods will be separated by a minimum 1 week washout period. One of the interventions consists of 4 days of daily supplementation with the commercially available broccoli sprout extract capsules (BrocElite). Another intervention will consist of 4 days of daily supervised treadmill running. The exercise protocol consists of 4 minutes of high-intensity running running followed by a 3 minute recovery period, repeated for a total of 4 repetitions. The last intervention combines the above two and consists of 4 days of daily supplementation and daily supervised exercise training. Before and after each intervention, basic anthropometrics (weight, heart rate, and blood pressure) will be recorded, and a venous blood sample will be collected. Blood samples will be analyzed for metabolic and inflammatory markers. Participants will be asked to refrain from structured exercise, alcohol consumption, and broccoli consumption during each of the experimental periods. Before any testing, willing participants will provide informed consent, and fill out a few demographic, and lifestyle questionnaires. The study will be conducted at the University of British Columbia in Kelowna, BC, Canada.
Interventions
DIETARY_SUPPLEMENTSulforaphane-rich Broccoli Sprout Extract
Broc Elite Plus stabilized sulforaphane capsules from Mara Labs
OTHERHigh Intensity Interval Treadmill Running
4 cycles of: 4 minutes of intense treadmill running (85-95% HR max) followed by 3 minutes of slow, easy running.
Sponsors
University of British Columbia
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Randomized-crossover design
Eligibility
Sex/Gender
ALL
Age
19 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* aged between 19 and 35 years * not a current smoker * physically active based on the Canadian Physical Activity Guidelines (150 minutes of weekly physical activity)
Exclusion criteria
* a history of cardiometabolic diseases or respiratory diseases (e.g., chronic obstructive pulmonary disease, asthma, diabetes, coronary artery disease) * currently following a ketogenic diet * body mass index (BMI) over 30 kg/m2 * unable to read or communicate in English * having received a vaccination or experienced an upper respiratory tract infection in the last 4 weeks * having donated more than 0.5 L of blood within the last 4 weeks * currently pregnant * have contraindications to high intensity exercise (assessed using the CSEP Get Active Questionnaire)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in peripheral blood mononuclear cell oxygen consumption rate | 0-4 days | OCR will be measured using a Seahorse Analyzer before and after each 4-day intervention |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| TNF-alpha secretion from LPS-stimulated whole blood | 0-4 days | TNF-alpha secretion from LPS-stimulated whole blood will be measured via ELISA before and after each 4-day period |
| Change in percentage of classical monocytes before and after the 6-week intervention | 0-4 days | The percentage of classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention |
| Change in percentage of intermediate monocytes before and after the 4-day intervention | 0-4 days | The percentage of intermediate monocytes will be measured using flow cytometry at baseline and after the 4-day intervention |
| Change in percentage of non-classical monocytes before and after the 4-day intervention | 0-4 days | The percentage of non-classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention |
| Change in TLR-4 expression on classical monocytes before and after the 4-day intervention | 0-4 days | TLR-4 expression on classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention. |
| Change in TLR-4 expression on intermediate monocytes before and after the 4-day intervention | 0-4 days | TLR-4 expression on intermediate monocytes will be measured using flow cytometry at baseline and after the 4-day intervention. |
| Change in TLR-4 expression on non-classical monocytes before and after the 4-day intervention | 0-4 days | TLR-4 expression on non-classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention |
| Change in white blood cell counts before and after the 4-day intervention | 0-4 days | White blood cell counts will be measured using a hematology analyzer at baseline and after the 4-day intervention |
| TNF-alpha secretion from PolyIC-stimulated whole blood | 0-4 days | TNF-alpha secretion from PolyIC-stimulated whole blood will be measured via ELISA before and after each 4-day period |
| Change in peripheral blood mononuclear cell extracellular acidification rate before and after the 4-day period | 0-4 days | PBMC ECAR will be measured using a Seahorse Analyzer at baseline and after the 4-day intervention |
| Change in PBMC mitotracker green staining before and after the 4-day period | 0-4 days | PBMC mitotracker green will be measured using flow cytometry before and after the 4-day intervention period |
| Change in PBMC mitoSOX red staining before and after the 4-day period | 0-4 days | PBMC mitoSOX red will be measured using flow cytometry before and after the 4-day intervention period |
| Change in PBMC mitochondrial respiration before and after the 4-day intervention period | 0-4 days | PBMC mitochondrial respiration will be measured using high-resolution respirometry before and after the 4-day period |
Countries
Canada
Contacts
CONTACTHashim Islam Principle Investigator, Ph.D
Outcome results
None listed