Circadian Rhythm
Conditions
Keywords
Chrononutrition, circadian clock, sleep, pistachio
Brief summary
The goal of this study is to evaluate the effect of pistachio (Pistacia vera L.) consumption on plasma melatonin, antioxidant capacity, and circadian rhythms in overweight young adults. The main question it aims to answer are: * Can the consumption of pistachios, specifically their content of compounds like phyto-melatonin, effectively regulate the human biological clock and circadian rhythms?
Detailed description
This study will provide scientific evidence supporting the biological clock-regulating action of compounds present in pistachios, such as phyto-melatonin, their impact on circadian rhythm regulation, and improvement of metabolic processes altered during obesity.
Interventions
OTHERPistacho supplementation
Participants consumed 28 g (equivalent to 5.6 mg of phyto-melatonin) of pistachios each day, specifically 40 pistachios with shells, 1 hour to 30 minutes before bedtime
Sponsors
Universidad Autonoma de Queretaro
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
A 28-day, uncontrolled, base-final intervention study
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* No diagnosed diseases * Not regularly taking medications that affect melatonin synthesis or body regulation.
Exclusion criteria
* Smokers * Pregnant women * Individuals with night shifts, those following special diets or taking weight loss supplements * People who had undergone recent surgical procedures or significant weight loss in two months before the study. * Those taking sleep medications. * Individuals who had made trans meridian trips within 1 month of the evaluation, * Individuals with allergies, intolerances, or aversions to nuts/pistachios * Participants who voluntarily withdrew from the study * Participants who tested positive for COVID-19 during the intervention * Participants who experienced adverse effects.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Body composition analysis | Baseline, 2 weeks, 4 weeks | Body composition analysis (BCA, including fat mass \[FM\] and fat-free mass \[FFM\], in kg and %) was performed with a seca mBCA 514/515 medical Body Composition Analyzer (Allers Hamburg, Germany). The measurements were conducted in accordance with the guidelines of the International Society for the Advancement of Kinanthropometry (ISAK). |
| Waist circumference | Baseline, 2 weeks, 4 weeks | Waist circumference (WC, in cm) was measured using an ergonomic SECA 201 measuring tape. |
| Plasma melatonin | Baseline, 2 weeks, 4 weeks | Plasma obtained from the same samples of biochemicals determinations. Plasma melatonin levels were measured before and after the intervention using a commercial plasma melatonin kit. |
| Circadian locomotor activity | Through study completion during 28-days. | A temperature sensor on the wirst, an activity and body position sensor was placed on the participant's arm and a luxometer was worn on the neck with a pendant over the clothing. |
| Questionnaire of activity during the day | Baseline, 2 weeks, 4 weeks | Horne and Östberg morning- evening test. Visual Analog Score with a cumulative score scale |
| Physical activity questionnaire (IPAQ) | Baseline, 2 weeks, 4 weeks | Measured with the scale MET-minutes per week" (o MET-min/week) |
| Pittsburgh Sleep Quality Index (PSQI) | Baseline, 2 weeks, 4 weeks | Cumulative score scale by Gobal PSQI score |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Concentration of metabolic and lipid parameters | Baseline, 2 weeks, 4 weeks | Metabolic parameters, including glucose (mg/dL), cholesterol (HDL-c and LDL-c, mg/dL), and triglycerides (mg/dL). |
| Plasma antioxidant activity by FRAP method | Baseline, 2 weeks, 4 weeks | The antioxidant activity in deproteinized plasma was assessed using the ferric reducing antioxidant power (FRAP) assay. Plasma antioxidant activity was measured as milimol equivalent of Trolox/mL. |
| Plasma antioxidant activity by ORAC assay. | Baseline, 2 weeks, 4 weeks | The antioxidant activity in deproteinized plasma was assessed using the oxygen radical absorbance capacity (ORAC) method." |
Countries
Mexico
Outcome results
None listed