Diet Induced Thermogenesis, Heat Stress, Protein Consumption
Conditions
Keywords
high protein diet, exercise, hot environment
Brief summary
This project examines whether consuming a high-protein or high-carbohydrate beverage before exercise in the heat impacts metabolism and heat balance during exercise, with the following research questions: 1. Does a high-protein vs. high-carbohydrate pre-exercise drink impact metabolism and body temperature responses during a treadmill walking in the heat? 2. Does a high-protein or high-carbohydrate drink change oxygen use and heat production during treadmill walking in the heat?
Interventions
DIETARY_SUPPLEMENTProtein shake
22 oz of a beverage containing 320 kilocalories (60g protein, 6g fat, 8g carbohydrate).
DIETARY_SUPPLEMENTCarbohydrate shake
22oz of a carbohydrate drink containing 320 kilocalories (75g carbohydrate, 3g protein, \<1g fat)
Sponsors
Providence College
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Intervention model description
Double-blind crossover design
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Able to walk or run up to 5 mph on a treadmill * Able to consume products containing dairy (no allergy or dietary restrictions)
Exclusion criteria
* Following a diet that excludes any macronutrient group (low-carb or low-fat) * Diagnosed cardiovascular or metabolic diseases (hypertension, diabetes) * Diagnosed heat illness in past 3 months (heat exhaustion, heat stroke)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Metabolic Heat Production | Measured during 60 minutes of steady state exercise in 36C, 40% humidity | Heat production measured during exercise at 6 watts/kg, calculated based on oxygen consumption and carbon dioxide production during exercise (indirect calorimetry) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Core Temperature | Measured at rest and following 60 minutes of exercise in a warm (36C, 40% humidity) environment | Internal temperature change during exercise |
| Urinary NGAL (ng/mL) | Following 60 minutes of exercise in a warm environment (36C, 40% relative humidity) | Measured after exercise in high-protein and high-carbohydrate trials, NGAL (neutrophil-gelatinase associated lipocalin; a marker of acute kidney injury) will be measured in urine samples in each trial to assess kidney function. |
| Urine Specific Gravity (USG) | Measured before and after 60 minutes of exercise in a warm (36C, 40%RH) environment. | Measured in urine samples provided before and after exercise, USG will be used as a marker of urine concentration and hydration status with each intervention. |
| Proteinuria | Following 60 minutes of exercise in a warm environment (36C, 40% relative humidity) | Measured after exercise in high-protein and high-carbohydrate trials, total protein content will be measured in urine samples in each trial to assess kidney function. |
Contacts
CONTACTBrett R Ely, PhD
CONTACTMargaret Morrissey-Basler, PhD
Outcome results
None listed