Bariatric Surgery, Eating Behavior, Laparoscopic Sleeve Gastrectomy, Nutritional Status, Obesity
Conditions
Keywords
Obesity, Bariatric Surgery, Laparoscopic Sleeve Gastrectomy, Eating Behavior, Nutritional status, Ghrelin, GLP-1, Peptite YY, Appetite hormones, Body mass index, Weight loss, Dietary intake
Brief summary
This study aims to evaluate changes in eating behavior attitudes, nutritional status, and appetite-regulating hormonal parameters in individuals who have undergone Laparoscopic Sleeve Gastrectomy (LSG). The study will be conducted at Bursa Yıldırım Doruk Hospital, Department of General Surgery. A total of 40 volunteer participants who underwent LSG within the last month will be included. Participants will be assessed at baseline (shortly after surgery) and at 6-month follow-up. Assessments will include anthropometric measurements (body weight, height, waist and hip circumference, BMI, waist-to-hip ratio), 3-day dietary intake records analyzed using BeBIS 8.2 software, and the Adult Eating Behavior Scale. Biochemical parameters including fasting blood glucose, total cholesterol, triglycerides, HDL, LDL, VLDL cholesterol, and insulin will be obtained from patient files and analyzed using Advia Chemistry 2400 and Advia XPT devices. Additionally, appetite-regulating hormones (ghrelin, GLP-1, PYY) will be analyzed from residual blood samples using the ELISA method with RelAssay Diagnostics kits by Teknogen Bioengineering Laboratory. All data will be compared between baseline and 6-month follow-up to evaluate the impact of bariatric surgery on nutritional and hormonal outcomes.
Detailed description
This observational study will be conducted at Bursa Yıldırım Doruk Hospital, General Surgery Clinic, under the supervision of Assoc. Prof. Dogukan Durak. Forty volunteer participants who underwent Laparoscopic Sleeve Gastrectomy (LSG) within the last month and meet the inclusion and exclusion criteria will be enrolled after signing an informed consent form. Assessments will be performed at two time points: at baseline (shortly after surgery) and at 6-month follow-up. Anthropometric Measurements: Body weight, height, waist and hip circumference will be measured. BMI and waist-to-hip ratio will be calculated accordingly. Waist circumference will be evaluated according to WHO criteria. Dietary Assessment: A 3-day dietary intake record will be collected from each participant at both time points. Food portion sizes will be estimated using the "Food and Nutrient Photo Catalog." Energy and macronutrient intake will be analyzed using BeBIS 8.2 software and evaluated according to the Turkish Dietary Guidelines 2022 (TUBER 2022). Eating Behavior Assessment: The Adult Eating Behavior Scale (developed by Celik et al.) will be administered at both time points. The scale consists of 18 items and 5 subscales, scored between 0-100. Higher scores indicate more positive attitudes toward eating behavior. Biochemical and Hormonal Parameters: After 12-hour fasting, the following biochemical parameters will be obtained from patient files: fasting blood glucose (FBG), total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, very low-density lipoprotein (VLDL) cholesterol, and insulin. Routine biochemical parameters and lipid profile will be analyzed spectrophotometrically using the Advia Chemistry 2400 device, while insulin will be measured by chemiluminescence using the Advia XPT device. Additionally, appetite-regulating hormones including ghrelin, glucagon-like peptide-1 (GLP-1), and peptide YY (PYY) will be analyzed from residual blood samples stored at -20°C by Teknogen Bioengineering Laboratory using the ELISA method with an automated microplate reader (Biotek TS800) and RelAssay Diagnostics commercial kits (Ankara, Turkey). All data will be compared between baseline and 6-month follow-up using appropriate statistical methods.
Interventions
OTHERAnthropometric Measurements and Dietary Assessment
Anthropometric measurements (body weight, height, waist and hip circumference, BMI, waist-to-hip ratio), 3-day dietary intake records, Adult Eating Behavior Scale, and biochemical and hormonal parameters (fasting blood glucose, lipid profile, insulin, ghrelin, GLP-1, PYY) will be assessed at baseline and at 6-month follow-up.
Sponsors
Özlem ÖZPAK AKKUŞ
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
No
Inclusion criteria
* Having undergone Laparoscopic Sleeve Gastrectomy (LSG) within the last month * Aged between 18-50 years * Signing the informed consent form * Having a Body Mass Index (BMI) of 40 kg/m2 or between 35-40 kg/m2 with obesity-related comorbidities * No diagnosis of depression * No eating behavior disorder * No psychiatric disorder
Exclusion criteria
* Not having undergone Laparoscopic Sleeve Gastrectomy (LSG) or not planning to undergo bariatric surgery * Not being in the age range of 18-50 years * Not signing the informed consent form * Having a Body Mass Index (BMI) greater than 40 kg/m2 or less than 35 kg/m2 without obesity-related comorbidities * Having a diagnosis of depression * Having an eating behavior disorder * Having a psychiatric disorder
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in Serum Ghrelin Concentration (pg/mL) | Baseline and 6 months after surgery | Change in serum ghrelin levels between baseline and 6-month follow-up, assessed by ELISA method. |
| Change in Serum GLP-1 Concentration (pmol/L) | Baseline and 6 months after surgery | Change in serum glucagon-like peptide-1 (GLP-1) levels between baseline and 6-month follow-up, assessed by ELISA method. |
| Change in Serum PYY Concentration (pg/mL) | Baseline and 6 months after surgery | Change in serum peptide YY (PYY) levels between baseline and 6-month follow-up, assessed by ELISA method. |
| Change in Adult Eating Behavior Scale Total Score (0-100) | Baseline and 6 months after surgery | Change in Adult Eating Behavior Scale total score between baseline and 6-month follow-up. Scale is scored between 0-100; higher scores indicate more positive eating behavior attitudes. |
| Change in Daily Energy Intake (kcal/day) | Baseline and 6 months after surgery | Change in daily energy intake assessed by 3-day dietary intake records at baseline and 6-month follow-up. Food portions will be estimated using the Food and Nutrient Photo Catalog. Data will be analyzed using BeBIS 8.2 software and evaluated according to Turkish Dietary Guidelines 2022. |
| Change in Daily Protein Intake (g/day) | Baseline and 6 months after surgery | Change in daily protein intake assessed by 3-day dietary intake records at baseline and 6-month follow-up. Food portions will be estimated using the Food and Nutrient Photo Catalog. Data will be analyzed using BeBIS 8.2 software and evaluated according to Turkish Dietary Guidelines 2022 |
| Change in Daily Carbohydrate Intake (g/day) | Baseline and 6 months after surgery | Change in daily carbohydrate intake assessed by 3-day dietary intake records at baseline and 6-month follow-up. Food portions will be estimated using the Food and Nutrient Photo Catalog. Data will be analyzed using BeBIS 8.2 software and evaluated according to Turkish Dietary Guidelines 2022. |
| Change in Daily Fat Intake (g/day) | Baseline and 6 months after surgery | Change in daily fat intake assessed by 3-day dietary intake records at baseline and 6-month follow-up. Food portions will be estimated using the Food and Nutrient Photo Catalog. Data will be analyzed using BeBIS 8.2 software and evaluated according to Turkish Dietary Guidelines 2022. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in Body Weight (kg) | Baseline and 6 months after surgery | Change in body weight between baseline and 6-month follow-up. |
| Change in Body Mass Index (kg/m²) | Baseline and 6 months after surgery | Change in BMI between baseline and 6-month follow-up. Weight and height will be combined to report BMI in kg/m². |
| Change in Waist Circumference (cm) | Baseline and 6 months after surgery | Change in waist circumference between baseline and 6-month follow-up. |
| Change in Hip Circumference (cm) | Baseline and 6 months after surgery | Change in hip circumference between baseline and 6-month follow-up. |
| Change in Waist-to-Hip Ratio | Baseline and 6 months after surgery | Change in waist-to-hip ratio between baseline and 6-month follow-up. Waist and hip circumference measurements will be combined to calculate the waist-to-hip ratio. |
| Change in Fasting Blood Glucose Concentration (mg/dL) | Baseline and 6 months after surgery | Change in fasting blood glucose levels between baseline and 6-month follow-up. |
| Change in Total Cholesterol Concentration (mg/dL) | Baseline and 6 months after surgery | Change in total cholesterol levels between baseline and 6-month follow-up. |
| Change in Triglyceride Concentration (mg/dL) | Baseline and 6 months after surgery | Change in triglyceride levels between baseline and 6-month follow-up. |
| Change in HDL Cholesterol Concentration (mg/dL) | Baseline and 6 months after surgery | Change in high-density lipoprotein (HDL) cholesterol levels between baseline and 6-month follow-up. |
| Change in LDL Cholesterol Concentration (mg/dL) | Baseline and 6 months after surgery | Change in low-density lipoprotein (LDL) cholesterol levels between baseline and 6-month follow-up. |
| Change in VLDL Cholesterol Concentration (mg/dL) | Baseline and 6 months after surgery | Change in very low-density lipoprotein (VLDL) cholesterol levels between baseline and 6-month follow-up. |
| Change in Fasting Insulin Concentration (µIU/mL) | Baseline and 6 months after surgery | Change in fasting insulin levels between baseline and 6-month follow-up. |
Countries
Turkey (Türkiye)
Contacts
CONTACTÖzlem ÖZPAK AKKUŞ, Assoc. Prof.
CONTACTDoğukan DURAK, Assoc. Prof.
PRINCIPAL_INVESTIGATORÖzlem ÖZPAK AKKUŞ, Assoc. Prof.
Toros University
Outcome results
None listed