Peritoneal Lavage
Conditions
Brief summary
The proposed clinical research project aims to explore the functional dynamics of innate immune cells in patients. The protocol includes collecting samples from episodes of inflammatory disease, from which fluid will be extracted. The sampling procedure will be carried out non-invasively for the patient. Following collection, the fluid and cells will be separated. The cytokine profile of the fluid will be analyzed using ELISA and flow cytometry to detect inflammatory cytokines in the patient. The isolated cells from the fluid will then be analyzed by flow cytometry and differentially identified based on the physiological state of the patient, as well as to identify the presence of inflammatory markers in these immune cells.
Interventions
PROCEDUREDigestive surgery
Routine digestive surgery
Sponsors
Centre Hospitalier Universitaire de Nice
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
* All patients undergoing digestive surgery from 18 to 80 years old.
Exclusion criteria
* Patients under immunomodulatory treatments
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Immune cells dynamic | At the inclusion | Number of cells in each immune cell population, characterization of the composition of each population based on cellular subsets, |
Countries
France
Contacts
CONTACTZakariya Caillot
CONTACTAndrea Chierici
Outcome results
None listed