Healthy
Conditions
Keywords
Eosinophils, smoking, time of day, blood, flow cytometry
Brief summary
The study investigates the effect of acute smoking and time of day on blood eosinophils in chronic smokers. Chronic cigarette smokers without chronic respiratory diseases smoking at least 8 cigarettes per day and never-smoking controls are examined. Blood eosinophil counts and surface molecules are measured at 8 and 12 am in both groups. In smokers, two measurements are performed (at least 4 weeks apart): one measurement with no smoking between 8 and 12 am, and another measurement with smoking of 8 cigarettes between 8 and 12 am.
Detailed description
20 chronic cigarette smokers without chronic respiratory diseases smoking at least 8 cigarettes per day and 20 never-smoking controls are recruited. Blood eosinophil counts and surface molecules are measured at 8 and 12 am in both groups. In smokers, two measurements are performed (at least 4 weeks apart, cross-over design): one measurement with no smoking between 8 and 12 am, and another measurement with smoking of 8 cigarettes between 8 and 12 am. The effects of the time of day (comparing 8 and 12 am) and the effects of acute smoking (comparing the effects of no smoking or acute smoking at 12 am) on blood eosinophil counts and surface molecules (using flow cytometry) are analyzed. Additional parameters examined: lung function, exhaled nitric oxide, total and specific IgE in serum.
Interventions
OTHERSmoking for 4 hours
Smoking of 8 cigarettes in 4 hours
Sponsors
Marek Lommatzsch
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Intervention: Smoking. Intervention: Time of day.
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy current smokers * Age between 18-50 years * Smoking of at least 8 cigarettes/day
Exclusion criteria
* Chronic respiratory diseases * Any treatment with corticosteroids or other immunosuppressants * Any acute illness
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Blood eosinophil counts | 8 am (baseline) and 12 am (follow-up after intervention) | Blood eosinophil counts in cells/µl blood |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Blood eosinophil surface molecules | 8 am (baseline) and 12 am (follow-up after intervention) | Blood eosinophil surface molecules (such as CD62L or CD294), measured using flow cytometry |
Countries
Germany
Contacts
CONTACTMarek Prof. Lommatzsch, Prof.
CONTACTJohann C. Virchow, Prof.
Outcome results
None listed