Diode Laser Therapy, Liquorice Nanogel, Periodontal Disease
Conditions
Keywords
Liquorice Nanogel, Diode Laser, Real time pcr, P.gingivalis, 16s gene
Brief summary
Evaluation of the effect of liquorice nanogel versus diode laser on periodontitis stage Ⅲ clinically and microbiologically by molecular analysis of porphyromonas gingivalis using real time PCR.
Detailed description
Quantitative analysis technique using real time pcr (polymerase chain reaction) was done to detect presence of porphyromonas gingivalis. Pocket depth (PD), Plaque index (PI), gingival index (GI) and Clinical attachment level (CAL) were measured before work and after 3 months postoperatively. Analysis was made from gingival crevicular fluid samples of the patients using gutta percha paper points.
Interventions
DRUGLiquorice Nanogel
Real Time pcr was chosen to detect 16s gene concentration for porphyromonas gingivalis bacteria that is specific for Periodontitis Stage 3.
DRUGdiode laser
Real time Pcr was used to detect gene concentration 16s specific for porphyromonas gingivalis
Sponsors
Al-Azhar University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
SINGLE (Investigator)
Intervention model description
Patient had Periodontitis stage III with pocket depth ≥ 6mm; indicated for periodontal surgery or CAL ≥ 5mm , Patient agreed to the surgical procedure and clinical trial, Age ranged between 18-50 years old. No previous drug was taken affecting bone metabolism , No Smokers Pregnant women and women with menopause were excluded. Patients with psychological problems were excluded. Patients with abnormal habits were excluded . Patients with medical conditions as Diabetes or Hypertension were excluded.
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
No
Inclusion criteria
1. Patient had Periodontitis stage III with pocket depth ≥ 6mm; indicated for periodontal surgery or CAL ≥ 5mm. 2. Patient agreed to the surgical procedure and clinical trial. 3. Age ranged between 18-50 years old.
Exclusion criteria
* 1: No previous drug was taken affecting bone metabolism. 2- Smoking. 3- Pregnant women and women with menopause. 4- Patients with psychological problems . 5- Patients with abnormal habits. 6- Patients with medical conditions as Diabetes or Hypertension.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Quantitative analysis using real time pcr | 3 months |
Countries
Egypt
Outcome results
None listed