Oxidative Stress, Periodontitis
Conditions
Keywords
Thioredoxin reductase 1, TrxR1
Brief summary
This study aims to investigate the role of the thioredoxin system in the development and progression of periodontitis. Researchers will examine the levels of specific antioxidant markers, such as thioredoxin reductase 1 (TrxR1), glutathione (GSH), and malondialdehyde (MDA), in gingival tissue samples collected from healthy individuals and patients with different stages of periodontal disease. The goal is to determine if TrxR1 levels can serve as a potential biomarker for diagnosing the severity and activity of periodontitis.
Interventions
OTHERGingival Tissue Analysis
Gingival tissue samples are collected from all participants. These samples are analyzed for Glutathione (GSH) and Malondialdehyde (MDA) levels using spectroscopic methods. Additionally, the expression of thioredoxin reductase 1 (TrxR1), thioredoxin (TXN), and thioredoxin-interacting protein (TXNIP) is determined through quantitative real-time PCR, western blot, and enzyme activity assays.
Sponsors
Ataturk University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* Being between the ages of 18 and 70. * Having no known systemic disease. * Non-smokers. * No history of medication or supplement use in the last 3 months. * For the Periodontitis groups: Interdental clinical attachment loss (CAL), probing pocket depth (PPD), and radiographic bone loss according to 2017 World Workshop criteria.
Exclusion criteria
* Smoking. * Pregnancy or breastfeeding. * Presence of a systemic disease (e.g., diabetes mellitus, hypertension, rheumatoid arthritis, cancer, thyroid disorders, kidney or lung diseases). * History of any periodontal surgical procedure within the last 6 months. * Use of medication or supplements within the last 3 months.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Thioredoxin Reductase 1 (TrxR1) mRNA Expression Levels | Baseline (at the time of tissue collection) | The expression levels of TrxR1 in gingival tissue are determined by quantitative real-time PCR (qPCR). This measure evaluates the potential of TrxR1 as a biomarker for periodontitis severity. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Thioredoxin (TXN) mRNA Expression Levels | Baseline (at the time of tissue collection) | Evaluation of TXN gene expression levels in gingival tissue samples using quantitative real-time PCR (qPCR) normalized to β-actin. |
| Thioredoxin-Interacting Protein (TXNIP) mRNA Expression Levels | Baseline (at the time of tissue collection) | Evaluation of TXNIP gene expression levels in gingival tissue samples using quantitative real-time PCR (qPCR) normalized to β-actin. Time Frame: Baseline (at the time of tissue collection) |
| Malondialdehyde (MDA) Levels. | Baseline (at the time of tissue collection). | MDA levels in gingival tissue homogenates measured using the thiobarbituric acid reactive substances (TBARS) assay to evaluate lipid peroxidation. |
| Glutathione (GSH) Levels. | Baseline (at the time of tissue collection). | GSH levels in gingival tissue homogenates measured using spectroscopic methods to evaluate antioxidant status. |
Countries
Turkey (Türkiye)
Outcome results
None listed